MDFI Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99750

Core Product Specifications and Parameters

Biological Background: MyoD Family Inhibitor (MDFI) Function and Localization

• MDFI (MyoD family inhibitor), also known as I-MF or I-mfa, is a MyoD family inhibitor that negatively regulates myogenic transcription factors by retaining them in the cytoplasm and interfering with DNA binding.
• Plays a critical role in cellular differentiation, particularly in trophoblast and chondrogenic lineages.
• Modulates Wnt signaling by binding to the axin complex and increasing free β-catenin levels, thereby affecting both canonical Wnt and JNK pathways.
• Regulates TCF7L1/TCF3 transcriptional activity by direct interaction, preventing its DNA-binding.
• Regulates the activity of mechanosensitive Piezo channels (PubMed:37590348). Related references: PMID:37590348
• Subcellular localization: predominantly cytoplasmic but can shuttle to the nucleus; presence in both nucleus and cytoplasm is noted.
• Keywords: Development protein, Differentiation, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-160 of human MDFI, targeting the N-terminal region. Consider this when interpreting detection in isoforms or homologs.
• For Western blot, a band of approximately 25 kDa is expected. Validate using appropriate lysates from cell lines or tissues expressing MDFI (e.g., muscle, trophoblast).
• For immunofluorescence/ICC, anticipate cytoplasmic and nuclear staining patterns; include appropriate controls.
• For ELISA, the recombinant protein immunogen can serve as a standard; ensure cross-reactivity matches the species of interest.
• Cross-reactivity is reported with human, mouse, and rat; however, always verify specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a comprehensive range of antibodies, antigens, and ancillary reagents for diagnostic development. The MDFI antibody targets a developmental and signaling regulator linked to myogenesis and Wnt pathways, ideal for research in cell differentiation and cancer. Beyond this product, we can support your projects with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk reagents, and rare target sourcing—ensuring you have the materials needed from concept to clinic.