MDA5 Rabbit Monoclonal Antibody for WB, ELISA - Q9BYX4

Core Product Specifications and Parameters

Biological Background: MDA5 Function and Localization

• Encodes interferon-induced helicase C domain-containing protein 1 (IFIH1), also known as MDA5, Helicard, and RLR-2. It is a cytoplasmic innate immune receptor that recognizes viral dsRNA and uncapped mRNA with 2'-O-methylation deficiency.
• Upon ligand binding, MDA5 interacts with MAVS on mitochondria, triggering TBK1/IKBKE-mediated phosphorylation and activation of IRF3/IRF7, leading to type I interferon and pro-inflammatory cytokine production. Related references: PMID:28594402, PMID:32169843, PMID:33727702
• Acts as a major sensor for picornaviruses (e.g., EMCV, rhinovirus), coronaviruses (including SARS-CoV-2), and other RNA viruses such as dengue and West Nile viruses. Related references: PMID:28606988, PMID:33440148, PMID:33514628
• Localizes to the cytoplasm, mitochondria, and nucleus, consistent with its role in viral RNA sensing and signal transduction.
• Belongs to the RIG-I-like receptor (RLR) family, containing two N-terminal CARD domains, a DExD/H-box helicase domain, and a C-terminal regulatory domain. The helicase domain binds RNA in an ATP-dependent manner.
• Post-translational modifications include phosphorylation and ubiquitination, which regulate its activity and stability.
• Widely expressed with higher levels in placenta, pancreas, and spleen; little in brain, testis, and lung.
• Disease-associated mutations in IFIH1 are linked to Aicardi-Goutières syndrome and diabetes mellitus, highlighting its role in autoimmunity.
• Additionally, MDA5 may play a role in growth inhibition and apoptosis in tumor cells and enhance natural killer cell function.

Experimental Guidance and Technical Tips

• The immunogen spans a C-terminal region (aa 900-1000) of human MDA5, which is conserved across human, mouse, and rat, supporting the reported cross-reactivity. Validate species-specific reactivity in relevant sample matrices.
• For Western blotting, the antibody may detect bands at ~117 kDa (full-length MDA5) and possibly at ~25 kDa; using a broad molecular weight range and positive controls such as interferon-treated HeLa or A549 cells is advisable.
• In ELISA, titrate the antibody against recombinant MDA5 or the immunizing peptide to determine optimal working concentrations; ensure proper blocking and washing steps.
• Since MDA5 is interferon-inducible, consider stimulating cells with IFN-alpha/beta or viral mimics (e.g., poly(I:C)) to upregulate target expression before lysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This MDA5 rabbit monoclonal antibody is part of our portfolio of high-quality immunological reagents for innate immunity and virology research. In addition to antibodies, we supply validated antibody pairs, bulk ancillary reagents, and specialized raw-material sourcing for rare targets, simplifying your assay development pipeline.