MCM4 Rabbit Polyclonal Antibody for WB and ELISA - P33991

Core Product Specifications and Parameters

Biological Background: MCM4 Function and Localization

• MCM4 is a component of the MCM2-7 complex, the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation. Related references: PMID:16899510, PMID:25661590, PMID:32453425
• As part of the CDC45-MCM-GINS (CMG) helicase, MCM4 contributes to unwinding template DNA during replication and forming the replisome. Related references: PMID:16899510, PMID:25661590, PMID:32453425
• The MCM2-7 ring ATPase activity involves a conserved arginine finger motif provided in trans from adjacent subunits, with six active sites contributing differentially. Related references: PMID:16899510, PMID:25661590, PMID:32453425
• MCM4 localizes to the nucleus and chromosomes, consistent with its role in DNA replication.
• The protein undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may regulate its activity.
• MCM4 is annotated with keywords such as ATP-binding, DNA-binding, helicase, hydrolase, and cell cycle, reflecting its enzymatic and regulatory roles.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 350–450 of human MCM4; consider sequence alignment when assessing cross-reactivity across species.
• For Western blotting, expect a band near 97 kDa and include human, mouse, or rat cell lysates as positive controls.
• For ELISA, validate antibody recognition using the immunizing peptide or recombinant MCM4 protein.
• Store and handle according to standard protocols for polyclonal antibodies to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For DNA replication and cell cycle targets such as MCM4, we offer scalable sourcing solutions and technical support to streamline assay development. Partner with us for efficient, one-stop access to the raw materials needed to advance your IVD projects from concept to clinic.