MCL1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q07820

Core Product Specifications and Parameters

Biological Background: MCL1 Function and Localization

• MCL1 (Induced myeloid leukemia cell differentiation protein Mcl-1, also known as Bcl2-L-3) is a member of the Bcl-2 protein family involved in apoptosis regulation. It exists as two main isoforms: isoform 1 (MCL1L) inhibits apoptosis, while isoform 2 (MCL1S) promotes it.
• The primary function of MCL1 is to regulate cell survival versus apoptosis, maintaining viability without affecting proliferation. Isoform 1 exerts anti-apoptotic effects by interacting with other apoptosis regulators.
• MCL1 is localized to multiple subcellular compartments including the membrane, cytoplasm, mitochondrion, and nucleus (nucleoplasm), enabling its involvement in diverse cellular processes.
• Post-translational modifications and processing include phosphorylation, ubiquitination (isopeptide bond), and proteolytic cleavage, as indicated by keywords such as "Phosphoprotein" and "Ubl conjugation".
• MCL1 plays a role in differentiation and development, as highlighted by keywords "Developmental protein" and "Differentiation".
• Alternative splicing generates functionally distinct isoforms, contributing to the dual pro- and anti-apoptotic activities.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 150-250 of human MCL1, which corresponds to a region conserved in both isoforms and species. This design may detect both pro- and anti-apoptotic isoforms.
• For Western blot (WB), confirm the expected band size of ~37 kDa in lysates from human and mouse cells; additional bands may indicate isoform-specific detection.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and fixation conditions based on sample type due to the multi-compartment localization.
• Validate specificity in apoptosis-related assays by comparing expression levels in treated vs. untreated cells, and consider using knockout or siRNA controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials for apoptosis research, including validated MCL1 antibodies, recombinant proteins, and ancillary reagents. With expertise in Bcl-2 family targets, we can supply bulk quantities and support custom development needs. This product empowers researchers in cell survival and cancer studies.