MCL1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q07820

Core Product Specifications and Parameters

Biological Background: Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 Function and Localization

• Isoform 1 of Mcl-1, also known as Bcl-2-like protein 3, inhibits apoptosis, while isoform 2 promotes apoptosis, highlighting a dual role in cell fate regulation.
• The protein is a key member of the Bcl-2 family and mediates survival signals by interacting with pro-apoptotic regulators such as BAX and BAK.
• Mcl-1 localizes to multiple cellular compartments including the cytoplasm, mitochondrial outer membrane, nucleoplasm, and other membrane structures, reflecting its dynamic role in apoptosis.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation, which regulate protein stability and function.
• Originally identified as an early induction gene during myeloid leukemia cell differentiation, Mcl-1 is critical for the maintenance of cell viability without promoting proliferation.
• Alternative splicing generates distinct isoforms with opposing functions, adding complexity to its regulatory network in development and disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human MCL1 (amino acids 1-327), which may generate antibodies recognizing both isoforms; consider validating isoform-specific detection in the relevant sample system.
• For Western blot, ensure thorough membrane blocking and optimize primary antibody dilution to minimize non-specific binding, given the polyclonal nature.
• In immunofluorescence, permeabilization conditions may affect signal distribution due to the protein’s multiple subcellular localizations; perform proper controls.
• ELISA application may require titration of both capture and detection antibodies, and cross-reactivity with mouse and rat MCL1 should be confirmed in your assay setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and custom monoclonal/polyclonal antibodies. For apoptosis and oncology research, we offer this anti-MCL1 polyclonal antibody alongside complementary reagents and technical consulting to support your assay development from concept to commercial validation.