MCF2 Rabbit Polyclonal Antibody for WB and ELISA - P10911

Core Product Specifications and Parameters

Biological Background: MCF2 (Proto-oncogene DBL) Function and Localization

• MCF2 encodes the proto-oncogene DBL, a guanine nucleotide exchange factor (GEF) that modulates Rho family GTPases (RHOA, RAC1, CDC42), with distinct isoform-specific activities.
• Multiple isoforms arise from alternative splicing: isoform 1 lacks activity toward RHOA, RAC1, and CDC42; isoform 2 has reduced CDC42 GEF activity; isoform 3 weakly activates RAC1 and CDC42; isoform 4 significantly activates RHOA and CDC42; and the truncated oncogenic DBL is active toward all three GTPases.
• DBL belongs to the DBL family of GEFs, sharing structural homology with proteins such as ARHGEF21, and functions as a guanine-nucleotide releasing factor critical for cytoskeletal reorganization and cell signaling.
• Subcellularly, DBL is found in the cytoplasm and at the membrane, reflecting its role in relaying signals from receptor activation to GTPase-mediated actin dynamics.
• The protein is classified as a phosphoprotein and is subject to proteomic identification, suggesting post-translational modifications that may regulate its activity.
• Tissue expression varies by isoform: isoform 1 is brain-specific; isoform 3 is detected in heart, kidney, spleen, liver, and testis; isoform 4 is expressed in brain, heart, kidney, testis, placenta, stomach, and peripheral blood; overall, DBL protein is detected in brain, heart, kidney, intestine, muscle, lung, and testis.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 872–1001 of human MCF2 (NP_001165347.1), a C-terminal region that may detect multiple isoforms; consider isoform-specific expression patterns when interpreting results.
• For Western blot, the predicted molecular weight of full-length DBL is approximately 108 kDa; alternative isoforms or post-translational modifications may produce additional bands—validate specificity with appropriate controls.
• This antibody is listed for ELISA applications; optimize coating conditions and detection parameters for your specific sample matrix.
• Cross-reactivity with mouse is reported; verify performance in your experimental system as reactivity may vary with sample preparation and tissue source.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material procurement, providing comprehensive access to validated antibodies and ancillary reagents. For researchers investigating Rho GTPase signaling and proto-oncogene DBL function, this anti-MCF2 polyclonal antibody complements our portfolio of monoclonal and polyclonal antibodies, matched antibody pairs, and bulk reagents. With a commitment to one-stop sourcing from concept to clinic, CamelBio also facilitates procurement of rare target materials, supporting your diagnostic and research programs with reliable supply chain solutions.