MCCC2 Rabbit mAb for WB, IHC-P, IF/ICC, ELISA - Q9HCC0

Core Product Specifications and Parameters

Biological Background: MCCC2 Function and Localization

• MCCC2 encodes the methylcrotonoyl-CoA carboxylase beta chain, also known as the non-biotin-containing carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase enzyme.
• The protein functions in the mitochondrial matrix, catalyzing the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step in leucine and isovaleric acid catabolism.
• Alternative splicing of the MCCC2 gene produces multiple isoforms, potentially modulating enzyme activity or subcellular localization.
• Post-translational acetylation may regulate MCCC2 function, as indicated by proteomics studies.
• As a key enzyme in branched-chain amino acid degradation, MCCC2 plays an essential role in energy metabolism and metabolic intermediate provision.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 284–563 of human MCCC2; ensure it aligns with your target region of interest.
• For Western blotting, a specific band near 61 kDa is expected in human, mouse, and rat samples; validate under your experimental conditions.
• For IHC-P and IF/ICC, consider mitochondrial localization; appropriate fixation/permeabilization and antigen retrieval methods are recommended.
• Confirm antibody performance in your specific assay system using appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research teams to provide a reliable supply of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. This MCCC2 antibody is a robust tool for studies on amino acid metabolism and related disorders. Let us help streamline your procurement from concept to clinic.