MBD6 Rabbit pAb for WB, ELISA - Q96DN6

Core Product Specifications and Parameters

Biological Background: MBD6 Function and Localization

• MBD6 (Methyl-CpG-binding domain protein 6) is a non-catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex [PMID:24634419]. Related references: PMID:24634419
• The PR-DUB complex specifically deubiquitinates histone H2A monoubiquitinated at Lys-120 (H2AK119ub1), and MBD6 is important for the stability of PR-DUB components and stimulating its ubiquitinase activity [PMID:36180891]. Related references: PMID:36180891
• As part of the PR-DUB complex, MBD6 associates with chromatin enriched in H3K4me1, H3K4me3, and H3K27Ac, but not H3K27me3, and localizes to distinct genomic regions compared to MBD5-containing complexes [PMID:36180891]. Related references: PMID:36180891
• Heterochromatin recruitment of MBD6 is not mediated by DNA methylation, indicating a methylation-independent chromatin targeting mechanism [PMID:20700456]. Related references: PMID:20700456
• The PR-DUB complex, via MBD6, regulates gene expression programs involved in development, cell communication, signaling, proliferation, and viability, and may promote cancer cell growth [PMID:36180891]. Related references: PMID:36180891
• MBD6 localizes to the nucleus and chromosomes, consistent with its role in chromatin regulation.
• Post-translational modifications include ubiquitination, as indicated by the Ubl conjugation pathway keyword.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 880-980 of human MBD6 (NP_443129.3), a region near the C-terminus; consider cross-species sequence conservation when working with mouse or rat samples.
• For Western blotting, the predicted molecular weight is 101 kDa; include appropriate molecular weight markers and consider the use of a high-molecular-weight transfer protocol for optimal detection.
• In ELISA, this polyclonal antibody may be used for capture or detection; titrate the antibody to determine the optimal working concentration for your specific assay format.
• As a polyclonal antibody, expect inherent batch-to-batch variability; perform appropriate controls and validation experiments in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD raw materials, offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents to support diagnostic development from concept to clinic. Our MBD6 rabbit polyclonal antibody is an example of the quality raw materials we provide for epigenetics and chromatin research applications. Whether you require validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare-target raw-material sourcing, CamelBio bridges your procurement needs with reliable supply solutions.