MBD2 Rabbit Polyclonal Antibody for WB and ELISA - Q9UBB5

Core Product Specifications and Parameters

Biological Background: MBD2 Function and Localization

• Specifically binds methylated CpG islands and hemimethylated DNA, enabling recognition of epigenetic marks. Related references: PMID:9774669, PMID:10947852, PMID:24307175
• Recruits histone deacetylases and DNA methyltransferases to chromatin, acting as a transcriptional repressor for gene silencing. Related references: PMID:10471499, PMID:10947852
• Integral component of the NuRD complex, participating in chromatin remodeling and transcription repression. Related references: PMID:16428440, PMID:28977666
• Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to mediate repressive activities. Related references: PMID:16415179
• May enhance activation of certain unmethylated cAMP-responsive promoters under specific conditions. Related references: PMID:12665568
• Localizes to the nucleus and associates with chromosomes, consistent with its role in transcriptional regulation.
• Highly expressed in brain, heart, kidney, stomach, testis, and placenta, suggesting tissue-specific functions.
• Post-translational modifications include phosphorylation, and alternative splicing produces distinct isoforms.

Experimental Guidance and Technical Tips

• Immunogen encompasses C-terminal residues 350-411, targeting a region likely involved in protein interactions; the antibody is expected to recognize full-length MBD2.
• For Western blotting, a band around 43 kDa is predicted; consider using nuclear extracts to enrich the target protein.
• When analyzing multiple species, include human, mouse, and rat positive controls to confirm cross-reactivity.
• For ELISA applications, optimize coating and detection conditions using recombinant MBD2 or nuclear lysates.
• Validate antibody performance in your specific experimental system, as detection may vary with sample preparation and isoform abundance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibodies for epigenetic targets like MBD2. Beyond this MBD2 polyclonal antibody, we offer matched antibody pairs, recombinant proteins, and bulk reagent supply to streamline your assay development from concept to clinic. Our sourcing expertise ensures reliable access to rare-target raw materials for your diagnostic needs.