MBD2 Rabbit pAb - Q9UBB5

Core Product Specifications and Parameters

Biological Background: MBD2 Function and Localization

• MBD2 is a methyl-CpG-binding domain protein that specifically recognizes and binds to methylated CpG dinucleotides in DNA, including hemimethylated DNA. Related references: PMID:9774669, PMID:10947852, PMID:24307175
• It functions as a transcriptional repressor by recruiting histone deacetylases and DNA methyltransferases to chromatin, contributing to gene silencing. Related references: PMID:10471499, PMID:10947852, PMID:16415179
• MBD2 is a core component of the NuRD (nucleosome remodeling and deacetylase) complex, which remodels chromatin to regulate transcription. Related references: PMID:16428440, PMID:28977666
• It acts as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. Related references: PMID:16415179
• MBD2 may also enhance activation of some unmethylated cAMP-responsive promoters. Related references: PMID:12665568
• Subcellular localization is predominantly nuclear, associated with chromosomes.
• It is highly expressed in brain, heart, kidney, stomach, testis, and placenta.
• Post‑translational modifications include phosphorylation, and alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant protein covering amino acids 202–411 of human MBD2; the polyclonal nature may recognize multiple epitopes and isoforms.
• For Western blot, a band of approximately 43 kDa is expected; verify detection in relevant human, mouse, and rat samples as cross‑reactivity is reported.
• In ELISA applications, optimize coating and detection conditions; the antibody may be used as a capture or detection reagent depending on assay design.
• Consider performing titration experiments to determine the optimal working concentration for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined, one‑stop sourcing experience for diagnostic manufacturers and research laboratories, providing validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents for IVD development. With expertise in chromatin biology targets like MBD2, CamelBio supports your projects from concept to clinic, including rare‑target raw‑material sourcing and technical consultation.