MAVS Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q7Z434

Core Product Specifications and Parameters

Biological Background: MAVS Function and Localization

• MAVS (Mitochondrial antiviral-signaling protein, also known as IPS-1, VISA, Cardif) is an adapter essential for innate immune defense against viruses, acting downstream of cytoplasmic RNA sensors RIG-I and MDA5 to activate NF-kappa-B and IRF3/IRF7 transcription factors, leading to induction of antiviral cytokines such as interferon-beta and RANTES. Related references: PMID:16125763 PMID:16127453 PMID:16153868

• Following viral infection, peroxisomal MAVS initiates a rapid, interferon-independent defense, while mitochondrial MAVS triggers a delayed, interferon-dependent amplification pathway to sustain the antiviral state. Related references: PMID:20451243

• MAVS also participates in NLRP3 inflammasome activation by mediating NLRP3 recruitment to mitochondria. Related references: PMID:23582325

• The protein localizes to the mitochondrial outer membrane, mitochondrion, and peroxisome.

• It is ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta, and peripheral blood leukocytes; at the protein level, it is detected in T-cells, monocytes, epithelial cells, and hepatocytes.

• MAVS undergoes diverse post-translational modifications, including acetylation, phosphorylation, ubiquitination, and palmitoylation, which are critical for its signaling functions.

• Alternative splicing generates multiple isoforms with potentially distinct regulatory roles.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a synthetic peptide spanning amino acids 1–100 of human MAVS, which includes the N-terminal CARD domain; verify reactivity with your target isoform if alternative splicing is a concern.
• For Western blot (WB), a band around 57 kDa is expected in human, mouse, and rat samples; consider testing in relevant virus-infected or stimulated cell lines (e.g., HEK293, HeLa, Huh7).
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), permeabilization and antigen retrieval conditions may require optimization for mitochondrial membrane proteins; including digitonin can improve mitochondrial staining.
• For ELISA applications, the antibody may be used for capture or detection, but dilution and pairing must be empirically optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive procurement partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This MAVS antibody supports researchers and diagnostic developers exploring antiviral innate immunity and related signaling pathways. Our team can also source rare targets and provide technical and supply chain support to accelerate your project from concept to clinic.