MAVS Rabbit mAb for WB, IHC-P, IF/ICC, ELISA - Q7Z434

Core Product Specifications and Parameters

Biological Background: MAVS Function and Localization

• MAVS (Mitochondrial antiviral-signaling protein), also known as IPS-1, VISA, and CARDIF, is a key adaptor in the innate immune response to viral infection.
• MAVS functions downstream of the cytosolic RNA sensors RIG-I and MDA5, coordinating signaling cascades that activate transcription factors NF-κB, IRF3, and IRF7, leading to the production of antiviral cytokines such as IFN-β and RANTES (CCL5).
  Related references:
  PMID:16125763 PMID:16127453 PMID:16153868
• It may also mediate antiviral signaling through TLR3 in response to extracellular dsRNA.
  Related references:
  PMID:16153868
• MAVS localizes to both mitochondria and peroxisomes, where it orchestrates temporally distinct antiviral responses: peroxisomal MAVS triggers rapid, interferon-independent defense, while mitochondrial MAVS drives a sustained, interferon-dependent amplification phase.
  Related references:
  PMID:20451243
• The protein exhibits anti-apoptotic activity, protecting cells from programmed cell death during viral infection.
  Related references:
  PMID:16125763
• MAVS participates in NLRP3 inflammasome activation by mediating the recruitment of NLRP3 to mitochondria.
  Related references:
  PMID:23582325
• Subcellularly, MAVS is an integral membrane protein residing on the mitochondrial outer membrane and peroxisomes, anchored via a C-terminal transmembrane helix.
• Ubiquitously expressed, MAVS is detected at high levels in heart, skeletal muscle, liver, placenta, and peripheral blood leukocytes; at the protein level, it is present in T-cells, monocytes, epithelial cells, and hepatocytes.
• Subject to diverse post‑translational modifications, including acetylation, alternative splicing, lipidation (palmitoylation), phosphorylation, and ubiquitination, which regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen used for this rabbit monoclonal antibody covers amino acids 121–220 within the N-terminal region of human MAVS, a domain important for its signaling adaptor function. Verify sequence identity across species to anticipate cross‑reactivity.
• For Western blotting, a band corresponding to the predicted molecular weight of ~57 kDa should be observed. Confirm by using appropriate positive controls (e.g., human or mouse cell lines stimulated with poly(I:C) or viral infection).
• In immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), mitochondrial and peroxisomal staining patterns may be visible; optimization of fixation and antigen retrieval is recommended. A punctate cytoplasmic distribution is expected.
• For ELISA applications, ensure the antibody recognizes the native or recombinant MAVS protein under the assay conditions, and titrate the antibody to determine optimal coating and detection concentrations.
• As a monoclonal antibody, it offers consistent lot‑to‑lot performance, minimizing variability in multi‑experiment studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes by providing verified IVD raw materials—including monoclonal and polyclonal antibodies, antibody pairs, and bulk ancillary reagents—for efficient assay development. The MAVS Rabbit mAb is a prime tool for studying innate antiviral immunity and inflammatory signaling pathways, facilitating infectious disease research and biomarker discovery. For rare‑target sourcing or customized production needs, CamelBio’s integrated technical services and consulting can help bridge the gap from concept to clinic.