MAT1A Rabbit pAb - Q00266

Core Product Specifications and Parameters

Biological Background: MAT1A Function and Localization

• MAT1A encodes S-adenosylmethionine synthase isoform type-1 (methionine adenosyltransferase 1), also designated MAT 1 or MAT-I/III, responsible for the synthesis of the primary methyl donor S-adenosylmethionine (AdoMet).
• The enzyme catalyzes AdoMet formation in two sequential steps: first, methionine and ATP yield AdoMet and triphosphate; second, triphosphate is hydrolyzed to pyrophosphate and phosphate.
• Tissue specificity reveals that MAT1A is predominantly expressed in the liver, underpinning its critical role in hepatic one-carbon metabolism.
• UniProt keywords classify MAT1A with ATP-binding, magnesium and potassium ion binding, S-nitrosylation, and disulfide bond features, indicating multiple regulatory modes.
• The reviewed molecular weight is 43.6 kDa, and the product immunogen covers amino acids 1-395, which includes the active site region.
• Proteomics identification and reference proteome status confirm MAT1A as a well-characterized human enzyme.

Experimental Guidance and Technical Tips

• Given the immunogen spans nearly the full-length protein (aa 1-395), affinity purification may yield polyclonal antibodies that recognize both native and denatured MAT1A; verify specificity in liver lysate western blots.
• For ELISA, coat recombinant MAT1A or synthetic peptide to establish a standard curve, and consider using a blocking buffer optimized for rabbit polyclonal antibodies.
• Cross-reactivity with mouse and rat extends potential use to rodent liver models; however, confirm antibody performance in these species by pilot experiments.
• The predicted molecular mass of 44 kDa should guide SDS-PAGE conditions; ensure adequate gel percentage for clear separation.

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