Mast Cell Tryptase Rabbit mAb for WB, IHC-P, IP, ELISA - Q15661

Core Product Specifications and Parameters

Biological Background: Mast Cell Tryptase Function and Localization

• Tryptase alpha/beta-1 (encoded by TPSAB1) is the major neutral serine protease stored in mast cell secretory granules, released upon mast cell activation and degranulation.
• Isoform 2 (also known as tryptase beta-1) cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates. Related references: PMID:18854315
• The TPSAB1 gene produces multiple isoforms (TPS1, TPS2, TPSB1, TPSB2) through alternative splicing, with tryptase-2 being one variant.
• Subcellular localization is predominantly secreted from mast cells into the extracellular space.
• Tissue expression: Isoform 1 is predominant in lung, stomach, spleen, heart, and skin; isoform 2 is expressed in aorta, spleen, breast tumor, with highest levels in endothelial cells of certain blood vessels, and low levels in mast cells.
• The protein features a signal peptide, undergoes glycosylation, and contains disulfide bonds, and is classified as a serine protease hydrolase and zymogen.
• Molecular weight of the mature protein is approximately 30.5 kDa, consistent with the observed band at 31 kDa in applications.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide, the antibody likely recognizes a linear epitope; denaturing conditions in WB may be suitable.
• For IHC-P, consider antigen retrieval methods appropriate for tryptase detection in mast cell-rich tissues like skin or lung.
• For IP, validate the antibody's ability to pull down the 31 kDa protein under non-denaturing conditions.
• Because of cross-reactivity with mouse, the antibody can be used in mouse models of allergy or mast cell biology, but verify species-specific reactivity.
• Note that tryptase may be released in soluble form; ELISA may require capture-detection optimization.
• The antibody is monoclonal, ensuring batch-to-batch consistency; always include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, providing validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to streamline your diagnostic development. For tryptase-related research or mast cell biology assays, we ensure consistent supply of high-quality raw materials to accelerate your project from concept to clinic.