MARCKSL1 Rabbit Polyclonal Antibody for WB and ELISA - P49006

Core Product Specifications and Parameters

Biological Background: MARCKSL1 Function and Localization

• MARCKSL1 (MARCKS-like protein 1, also known as Mac-MARCKS, F52, MLP, MRP, MLP1, MACMARCKS) controls cell movement by regulating actin cytoskeleton homeostasis and the formation of filopodia and lamellipodia. Related references: PMID:22751924

• In its unphosphorylated state, the protein promotes cell migration; upon phosphorylation by MAPK8 (JNK), it induces actin bundle stabilization, reducing actin plasticity and restricting cell movement, including neuronal migration.

• May couple protein kinase C and calmodulin signal transduction systems.

• Localizes to the cytoplasm, cytoskeleton, and cell membrane, consistent with its role in actin dynamics.

• Contains actin-binding and calmodulin-binding domains, and a myristoylation site, characteristic of the MARCKS family of myristoylated alanine-rich protein kinase C substrates.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human MARCKSL1 protein (aa 1-195), suggesting the antibody may recognize multiple epitopes; it is suitable for Western blot (WB) and ELISA applications as per supplier specifications.
• In WB, a specific band is expected at approximately 20 kDa (predicted molecular weight 19529 Da). Validate protein loading and transfer using appropriate controls.
• This antibody shows cross-reactivity with human, mouse, and rat samples; consider species-specific validation when switching model systems.
• For ELISA, the polyclonal nature may provide broad antigen capture; optimize coating and detection conditions in your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-MARCKSL1 polyclonal antibody is part of our extensive catalog of validated research and diagnostic reagents, supporting studies on cytoskeleton organization and cell migration. We can also assist with sourcing bulk quantities, custom antibody development, and other rare-target raw materials to accelerate your IVD projects.