MAP7D1 Rabbit pAb for WB, ELISA - Q3KQU3

Core Product Specifications and Parameters

Biological Background: MAP7D1 Function and Localization

• MAP7 domain-containing protein 1 (MAP7D1), also known as arginine/proline-rich coiled-coil domain-containing protein 1 (RPRC1) or proline/arginine-rich coiled-coil domain-containing protein 1 (PARCC1), is encoded by the MAP7D1 gene (GeneID: 55700) in humans.
• MAP7D1 is a microtubule-stabilizing protein that plays a critical role in the control of cell motility and neurite outgrowth by maintaining acetylated stable microtubules.
• The protein belongs to the MAP7 family and contains coiled-coil domains, contributing to its structural and functional roles in cytoskeletal dynamics.
• MAP7D1 localizes to the cytoplasm and associates with various cytoskeletal structures, including the spindle, microtubule organizing center (centrosome), and midbody during cell division.
• Post-translational modifications of MAP7D1 include phosphorylation and ubiquitin-like (Ubl) conjugation (isopeptide bond formation), as identified by proteomics.
• Alternative splicing of the MAP7D1 gene generates multiple transcript variants, potentially diversifying the functional repertoire of the protein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200–460 of human MAP7D1, which includes a region likely to recognize the native protein. Confirm specificity by western blot at the expected molecular weight (~93 kDa) in appropriate lysates.
• For western blot (WB), use standard SDS-PAGE and transfer protocols; consider a positive control such as HeLa or SH-SY5Y cell lysates, as MAP7D1 is expressed in human and mouse.
• ELISA application may utilize recombinant MAP7D1 protein or peptide coating; validate assay conditions with known positive and negative controls.
• Due to cross-reactivity with mouse, this antibody can be employed in mouse models; however, pre-validate in the specific tissue or cell type of interest.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers diagnostic manufacturers and researchers a streamlined sourcing solution for high-quality IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents. This MAP7D1 polyclonal antibody supports research on microtubule dynamics and neuronal biology, and our team can assist with bulk orders, custom formulations, or complementary reagents like recombinant proteins. With end-to-end support from concept to clinic, CamelBio is your reliable partner for rare-target antibody sourcing and IVD development.