MAP2 Rabbit mAb - P11137

Core Product Specifications and Parameters

Biological Background: MAP2 Function and Localization

• Microtubule-associated protein 2 (MAP2) is a major microtubule-associated protein with a canonical molecular weight of ~199.5 kDa.
• The protein undergoes alternative splicing, producing isoforms MAP2A, MAP2B, and MAP2C, as indicated by the aliases MAP-2, MAP2A, MAP2B, and MAP2C.
• MAP2 localizes predominantly to the cytoplasm and cytoskeleton, with specific enrichment in dendritic cell projections.
• While its precise role is not fully defined, MAP2 is thought to stabilize microtubules against depolymerization and may confer mechanical stiffness to microtubules.
• MAP2 contains calmodulin-binding domains and is classified as a phosphoprotein, suggesting regulation by calcium signaling pathways and phosphorylation.
• The protein is a reference proteome component and has been identified in proteomics studies, underscoring its biological significance.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–118) targets the N-terminal region, which may be conserved across isoforms; validate isoform specificity in the relevant cell or tissue system if required.
• For western blot, anticipate a band around 200 kDa; use low-percentage SDS-PAGE gels and optimize transfer conditions for high molecular weight proteins.
• In immunofluorescence applications, consider fixation and permeabilization protocols that preserve microtubule architecture.
• For IHC-P, evaluate antigen retrieval methods and, if using biotin-based detection, account for endogenous biotin in neuronal tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research manufacturers with streamlined access to premium IVD raw materials, including validated MAP2 monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents. Specializing in neuronal targets and rare cytoskeletal proteins, our team supports development from concept to clinic with technical sourcing expertise. Partner with CamelBio to secure high-performance antibodies for immunoassays, IHC, and other clinical applications.