Mannose Receptor/CD206 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q61830

Core Product Specifications and Parameters

Biological Background: Mannose Receptor/CD206 Function and Localization

• Macrophage mannose receptor 1 (MRC1), also known as CD206, is a type I transmembrane glycoprotein and a member of the C-type lectin superfamily. It is encoded by the Mrc1 gene in mice (UniProt Q61830).
• MRC1 functions as a phagocytic and endocytic pattern recognition receptor, mediating the uptake of glycoproteins and pathogens by macrophages. It binds both sulfated and non-sulfated polysaccharide chains, facilitating clearance of mannose-/fucose-/N-acetylglucosamine-terminated ligands.
• The receptor plays a critical role in innate immunity by acting as a phagocytic receptor for bacteria, fungi, and other pathogens, thereby contributing to host defense and antigen presentation.
• Subcellularly, MRC1 cycles between the cell membrane and endosomal compartments, consistent with its role in endocytosis and phagocytosis; it is localized to the endosome membrane and cell surface.
• Tissue expression is predominantly detected in macrophages, aligning with its function in immune surveillance and pathogen recognition.
• Structural keywords from UniProt include 3D-structure (crystal structure of its C-type lectin domains), calcium-dependent binding (lectin activity), disulfide bond stabilization, glycosylation, a signal peptide, a transmembrane helix, and internal repeats characteristic of the mannose receptor family.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide around the C-terminal region of mouse MRC1; the antibody is expected to recognize the C-terminus, which may be advantageous for detecting full-length protein and avoiding steric hindrance from ligand-binding domains in functional studies.
• For Western blot (WB), anticipate a band at approximately 166 kDa. Reduce potential non-specific binding by using appropriate blocking agents and verify with controls, as high molecular weight proteins may show cross-reactive bands.
• In immunofluorescence (IF) and immunohistochemistry (IHC), note the membrane and endosomal localization; permeabilization may be needed for intracellular pool detection. Consider antigen retrieval for paraffin-embedded sections (IHC-P) and validate in the relevant sample system (human, mouse, rat).
• ELISA applications can be optimized by pairing with an appropriate capture antibody or antigen coat; confirm cross-reactivity across species in the assay format of interest.
• Given the receptor’s role in pathogen phagocytosis, functional studies in macrophages may benefit from assessing antibody effects on ligand uptake or blocking assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined supply chain for IVD raw materials, connecting diagnostic manufacturers and research laboratories with validated antibodies, such as this anti-Mannose Receptor/CD206 rabbit mAb. For projects targeting macrophage biology, infectious diseases, or immune recognition assays, we can source optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare-target raw materials to support your entire development workflow—from concept to clinic.