MAN1A2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O60476

Core Product Specifications and Parameters

Biological Background: MAN1A2 Function and Localization

• MAN1A2, also known as mannosidase alpha class 1A member 2 or alpha-1,2-mannosidase IB, is a type II transmembrane protein encoded by the MAN1A2 gene (also designated MAN1B).
• It functions as a Golgi-resident glycosyl hydrolase that progressively trims α-1,2-linked mannose residues from the high-mannose oligosaccharide precursor Man9GlcNAc2 to yield Man5GlcNAc2, a key step in N-glycan maturation.
• Belongs to the glycosyl hydrolase 47 family, a group of α-1,2-mannosidases involved in protein quality control and endoplasmic reticulum-associated degradation.
• Localizes to the Golgi apparatus membrane, anchored via a single N-terminal transmembrane helix with a luminal catalytic domain.
• Post-translational modifications include acetylation and disulfide bonds; the protein binds calcium, which is essential for its catalytic activity and structural stability.
• Highest expression is observed in placenta and testis, suggesting roles in glycoprotein processing specific to these tissues.
• Identified in proteomics studies, indicating its potential involvement in broader cellular pathways.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 60-180, a region within the N-terminal domain; while this may provide specificity for MAN1A2, cross-reactivity with other mannosidases should be ruled out using appropriate controls.
• For western blotting, expect a band around 73 kDa; human placenta or testis lysates can serve as positive controls.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval optimization may be necessary; verify staining in tissues with known high expression, such as placenta or testis.
• The antibody is suitable for ELISA; recombinant MAN1A2 protein can be utilized as a standard for assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop portfolio of IVD raw materials, including validated monoclonal and polyclonal antibodies, recombinant proteins, and ancillary reagents, to accelerate your glycosylation-related research and diagnostic assay development. For your MAN1A2 projects, we supply high-quality antibody materials and can assist with sourcing rare targets and bulk reagents, bridging the gap from concept to clinical application.