Mammaglobin A Rabbit mAb - Q13296

Core Product Specifications and Parameters

Biological Background: Mammaglobin-A Function and Localization

• Mammaglobin-A (also known as Mammaglobin-1, Secretoglobin family 2A member 2) is a secreted protein encoded by the SCGB2A2 gene (gene aliases MGB1, UGB2).
• It belongs to the secretoglobin family, a group of small, secreted proteins with diverse functions.
• Subcellular localization: secreted extracellularly.
• Tissue specificity: highly specific to mammary gland tissue; expression is markedly over-expressed in breast cancers.
• Post-translational modifications: predicted to be a glycoprotein with a signal peptide for secretion; alternative splicing isoforms may exist.
• The predicted molecular weight is approximately 10 kDa (UniProt calculated mass: 10499 Da), consistent with the observed band size of 10 kDa.

Experimental Guidance and Technical Tips

• The immunogen used to raise this rabbit monoclonal antibody corresponds to the N-terminal region (amino acids 1–93) of human Mammaglobin-A.
• For Western blot (WB), use reducing and denaturing electrophoresis conditions; the small size (10 kDa) may require optimized transfer conditions (e.g., lower methanol percentage or shorter transfer time) and appropriate molecular weight markers for small proteins.
• For immunohistochemistry (IHC-P), consider antigen retrieval techniques and validate the antibody in known mammaglobin-positive breast cancer tissue sections. Include appropriate negative controls.
• For ELISA applications, the antibody may be used as either a capture or detection reagent; for sandwich ELISA, pairing with a second mammaglobin-specific clone recognizing a distinct epitope is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies this Mammaglobin A antibody as part of a comprehensive range of IVD raw materials, including validated antibody pairs, bulk monoclonal antibodies, and ancillary reagents for diagnostic kit development. With expertise in breast cancer biomarker sourcing, we help diagnostic manufacturers rapidly access high-quality materials and technical support from concept to clinic.