MAGOH Rabbit pAb - P61326

Core Product Specifications and Parameters

Biological Background: MAGOH Function and Localization

• MAGOH (Protein mago nashi homolog), also known as MAGOH1 or MAGOHA, is encoded by the MAGOH gene in humans.
• It is required for pre-mRNA splicing as a component of the spliceosome. Related references: PMID:11991638
• MAGOH plays a redundant role with MAGOHB as a core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway. The EJC marks the exon-exon junction and influences downstream processes including nuclear export, subcellular localization, translation efficiency, and NMD. Related references: PMID:23917022
• The MAGOH-RBM8A heterodimer inhibits EIF4A3 ATPase activity, stabilizing the EJC onto spliced mRNA. Interaction with PYM1 triggers EJC disassembly and enhances translation by recruiting spliced mRNAs to the ribosome.
• MAGOH modulates splicing of BCL2L1/Bcl-X, specifically inhibiting proapoptotic isoforms such as Bcl-X(S), distinct from its canonical EJC role.
• Subcellularly, MAGOH localizes to the nucleus (including nuclear speckles) and cytoplasm.
• It is ubiquitously expressed and associated with mRNA processing, splicing, transport, and translation regulation; post-translational acetylation has been reported.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1-146 of human MAGOH, which may help detect endogenous MAGOH across human, mouse, and rat due to sequence conservation.
• Suitable for Western blot (predicted molecular weight ~17 kDa) and ELISA. Consider optimizing antibody dilution for specific sample types and assay conditions.
• Since MAGOH is a nuclear/cytoplasmic protein involved in splicing, appropriate fractionation or localization studies may be considered.
• Validate the antibody in your relevant sample system to confirm specificity and performance.

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