MAD2B/MAD2L2 Rabbit mAb for WB, IF/ICC, ELISA - Q9UI95

Core Product Specifications and Parameters

Biological Background: MAD2B/MAD2L2 Function and Localization

• MAD2B/MAD2L2 (also known as REV7, FANCV) is an adapter protein involved in multiple cellular processes including translesion DNA synthesis, DNA double-strand break repair, cell cycle regulation, and transcription regulation. Related references: PMID:11459825, PMID:11459826, PMID:17296730
• It mediates the second polymerase switch during translesion DNA synthesis by linking the catalytic subunit REV3L of DNA polymerase zeta with the inserter polymerase REV1, allowing replication past DNA lesions. Related references: PMID:20164194, PMID:23143872
• As a component of the shieldin complex, MAD2B/MAD2L2 promotes non-homologous end joining (NHEJ) and suppresses DNA end resection during G1 and S phase, contributing to repair of DNA double-stranded breaks and maintaining genomic stability. Related references: PMID:29656893
• MAD2B/MAD2L2 inhibits the anaphase promoting complex (APC) by binding to FZR1 and possibly CDC20, thereby regulating mitotic progression and cell cycle checkpoints. Related references: PMID:11459825, PMID:17719540
• It also plays a role in transcription regulation by modulating TCF7L2-mediated gene expression and may be involved in epithelial-mesenchymal transdifferentiation. Related references: PMID:19443654
• The protein is widely expressed across tissues and localizes to the nucleus, cytoplasm, spindle apparatus, and chromosomes, consistent with its diverse functional roles.
• Key features include a conserved HORMA domain mediating protein-protein interactions; disease association with Fanconi anemia (FANCV) and involvement in DNA damage response highlight its physiological importance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100–200 of human MAD2B/MAD2L2 (Q9UI95); consider verifying reactivity in your specific model system.
• For Western Blot, expect a band at approximately 24 kDa; validate in lysates from human, mouse, or rat samples based on reported cross-reactivity.
• In immunofluorescence (IF/ICC), examine localization to the nucleus, cytoplasm, and spindle/centrosomal regions; optimize fixation and permeabilization conditions.
• ELISA applications may be suitable for detecting native or denatured protein; consider using the antibody as a capture or detection reagent in matched-pair assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research laboratories, offering one-stop access to validated IVD raw materials including antibodies like this MAD2B/MAD2L2 rabbit mAb. With expertise in DNA damage and cell cycle targets, we supply optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing support for rare targets, helping streamline your research and assay development from concept to clinic.