M1-linkage Specific Polyubiquitin Rabbit Polyclonal Antibody for WB, DB, and ELISA Applications

Core Product Specifications and Parameters

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (aa 1-100) of human ubiquitin, corresponding to reference sequences NP_066289.3 and NP_061828.1. Consider that the epitope may be specific to linear M1-linked polyubiquitin chains.
• For western blot experiments, use reducing and denaturing sample preparation to ensure epitope accessibility. Optimize antibody concentration for your specific sample type.
• In dot blot assays, validate specificity by including recombinant M1-linked ubiquitin chains and other linkage types (e.g., K48, K63) as controls to assess cross-reactivity.
• For ELISA, test different coating buffers and blocking agents to maximize signal-to-noise ratio. Verify performance with your target antigen.
• Cross-reactivity is indicated for human, mouse, and rat; perform parallel species-specific validation in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic sourcing partner for IVD manufacturers and research labs, providing consolidated access to high-quality raw materials like this M1-linkage specific polyubiquitin antibody. We supply validated antibodies, matched pairs, and ancillary reagents from pilot to bulk scales, including support for rare target acquisition. Our portfolio facilitates ubiquitin pathway research and diagnostic assay development with flexible, reliable procurement solutions.