Lysozyme (LYZ) Rabbit Polyclonal Antibody - P61626

Core Product Specifications and Parameters

Biological Background: Lysozyme Function and Localization

• Lysozyme C is a bacteriolytic enzyme that hydrolyzes 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine in peptidoglycan, compromising bacterial cell wall integrity.
• Secreted by various cells, including macrophages and Paneth cells, lysozyme is found in tears, saliva, human milk, and mucus, contributing to innate immune defense.
• It associates with the monocyte-macrophage system and enhances the activity of immunoagents, linking innate and adaptive immunity.
• Lysozyme belongs to the glycosyl hydrolase 22 family and is characterized by a highly conserved structure stabilized by four disulfide bonds.
• Mutations in the LYZ gene are linked to hereditary systemic amyloidosis (amyloidosis type VIII), where variant lysozyme forms amyloid fibrils that deposit in tissues.
• The protein undergoes direct protein sequencing, confirming its identity as a well-characterized reference proteome component.
• Lysozyme is a small protein of approximately 16.5 kDa with a signal peptide for secretion.
• Its antimicrobial role extends to antiviral and antifungal activities, making it a multifunctional host defense molecule.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-100 of human lysozyme C (NP_000230.1); this N-terminal region includes part of the mature protein and the signal peptide, so the antibody may recognize precursor and mature forms.
• For Western blotting, a band around 14-17 kDa is expected; use high-percentage gels (e.g., 15% SDS-PAGE) and optimize transfer conditions for small proteins.
• In immunohistochemistry (IHC-P), lysozyme exhibits granular cytoplasmic staining in macrophages and Paneth cells; consider antigen retrieval (e.g., citrate buffer, pH 6.0) to enhance signal.
• For immunofluorescence (IF/ICC), include a permeabilization step (e.g., 0.1% Triton X-100) to access intracellular lysozyme; note that secreted pools may require pre-extraction controls.
• For ELISA, the polyclonal format allows flexible assay design; validate as a capture or detection reagent by pairing with another clone or a polyclonal from a different host in the relevant sample matrix.
• Cross-reactivity is confirmed for human and mouse, but due to sequence conservation, reactivity with other species is possible; verify in the target species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For lysozyme-related biomarker and antimicrobial research, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support assay development. Our sourcing expertise enables the efficient procurement of rare-target raw materials, accelerating your diagnostic and translational projects.