LYAR Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NX58

Core Product Specifications and Parameters

Biological Background: LYAR Function and Localization

• LYAR (cell growth-regulating nucleolar protein, also known as ZLYAR or ZC2HC2) is a zinc-finger-containing protein with a predicted molecular weight of ~44 kDa; it functions as a DNA-binding transcriptional regulator.
• Plays a central role in pre-rRNA processing: maintains appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent conversion to mature 18S and 28S rRNAs (PubMed:24495227). Related references: PMID:24495227
• Acts as a transcriptional repressor: together with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression; preferentially binds the DNA motif 5'-GGTTAT-3' (PubMed:25092918). Related references: PMID:25092918
• In neuroblastoma cells, represses the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 (PubMed:28686580). Related references: PMID:28686580
• Negatively regulates the innate immune response by impairing IRF3 DNA-binding activity and inhibiting NF-kappa-B-mediated expression of pro-inflammatory cytokines (PubMed:31413131). Related references: PMID:31413131
• Localizes to the nucleus, nucleolus, cytoplasm, and cell projection cilium photoreceptor outer segment; predominantly expressed in testis.
• Additional functional roles: stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells, and maintains nucleolin (NCL) protein stability in embryonic stem cells to support self-renewal (By similarity).

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of LYAR is 44 kDa; validate the observed band size in your sample system, as post-translational modifications or isoforms may cause shifts.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), use appropriate antigen retrieval and blocking steps; expect staining in nucleus/nucleolus and cytoplasm, reflecting LYAR localization patterns.
• For ELISA applications, the recombinant immunogen (amino acids 10-160) can be used as a positive control; consider knockout or knockdown validation to confirm antibody specificity.
• Since this is a rabbit polyclonal antibody, pair with suitable anti-rabbit secondary reagents; appropriate isotype controls are recommended for immunostaining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a comprehensive sourcing solution for IVD raw materials, including validated polyclonal antibodies like this anti-LYAR antibody for studying nucleolar biology and transcriptional regulation. Our portfolio spans optimized antibody pairs, bulk ancillary reagents, and access to rare-target raw materials, streamlining your path from research to clinical application. For tailored procurement support and technical consultation, contact CamelBio to bridge your development needs efficiently.