Ly-6G Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P35461

Core Product Specifications and Parameters

Biological Background: Ly-6G Function and Localization

• Lymphocyte antigen 6G (Ly-6G), also known as Ly-6G.1 or Gr1, is a mouse protein encoded by the Ly6g gene (GeneID: 546644).
• It belongs to the Ly-6/uPAR superfamily of GPI-anchored cell surface proteins, characterized by a conserved LU domain.
• Subcellular localization is exclusively at the cell membrane, attached via a C-terminal GPI anchor.
• Tissue expression is predominantly observed in bone marrow, consistent with its role as a myeloid lineage marker.
• Key structural features include an N-terminal signal peptide, multiple disulfide bonds for LU domain stabilization, and N-linked glycosylation sites.
• The mature protein has a calculated molecular weight of 14,324 Da, consistent with the observed 14 kDa in reduced SDS-PAGE.
• UniProt keywords highlight its nature as a glycoprotein, lipoprotein, and membrane-anchored protein with a GPI modification.
• While specific molecular functions are not detailed in the UniProt record, Ly-6G is widely used as a neutrophil and myeloid-derived suppressor cell marker in mouse immunology.

Experimental Guidance and Technical Tips

• The immunogen covers the mature extracellular domain (aa 27–105), likely exposing epitopes accessible in native conformation; ideal for detecting surface Ly-6G by flow cytometry or IF on live cells.
• For western blotting (WB), a band around 14 kDa under reducing conditions is expected; non-reducing conditions may reveal dimeric forms due to disulfide bonds.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), antigen retrieval methods should be optimized, and endogenous peroxidase blocking is recommended for peroxidase-based detection.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with another clone may enhance sensitivity in sandwich formats.
• Always include proper tissue controls (e.g., mouse bone marrow) and isotype controls to validate specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD raw material procurement by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our Ly-6G monoclonal antibody supports mouse immunology and myeloid cell research, and we can source rare targets or custom formulations through our integrated supply chain. From concept to clinic, CamelBio bridges your diagnostic development needs with reliable, research-grade materials and technical consulting.