Ly-6C Rabbit pAb for WB, ELISA - P0CW02

Core Product Specifications and Parameters

Biological Background: Lymphocyte antigen 6C1 Function and Localization

• Lymphocyte antigen 6C1 (Ly-6C, also known as Ly6c; Ly-6C; Ly-6C1) is a mouse protein encoded by the Ly6c1 gene (Gene ID: 17067).
• It is a glycoprotein containing disulfide bonds, as indicated by UniProt keywords.
• The protein is attached to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor, classifying it as a lipoprotein (Keywords: GPI-anchor, Lipoprotein).
• It localizes to the cell membrane (SUBCELLULAR LOCATION: Cell membrane).
• The predicted molecular weight is ~14 kDa (UniProt: 14193 Da; product: 14KDa).
• A signal peptide is cleaved during maturation (Keyword: Signal).

Experimental Guidance and Technical Tips

• For Western blot under reducing conditions, expect a band around 14 kDa; use mouse lymphocyte or spleen lysates as positive control.
• Optimize antibody dilution for ELISA; checkerboard titration is recommended to determine the optimal coating antigen concentration and antibody dilution.
• Because this is a polyclonal antibody, validate lot-to-lot consistency if used in quantitative assays.
• The recombinant protein immunogen ensures high specificity, but cross-adsorption may be considered to reduce background if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions, including validated antibodies, antigen/immunogen design, and assay development support from concept to clinic. With expertise in immunological targets like Ly-6C, we source high-quality polyclonal antibodies tailored for diagnostic manufacturers and research laboratories, backed by reliable supply chains and technical consultation.