Ly-6A/E (Sca-1) Rabbit pAb - P05533

Core Product Specifications and Parameters

Biological Background: Ly-6A/E (Sca-1) Function and Localization

• Lymphocyte antigen 6A-2/6E-1, also known as stem cell antigen 1 (Sca-1) and T-cell-activating protein (TAP), is a mouse cell surface protein.
• It functions in T-cell activation, serving as a co-stimulatory molecule involved in lymphocyte signaling.
• The protein is attached to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor.
• Ly-6A/E is widely expressed in mouse tissues, including hematopoietic stem cells and lymphocytes.
• Post-translational modifications include a GPI-anchor, disulfide bond formation, and glycosylation.
• The molecular weight of the mature protein is approximately 14.4 kDa (calculated from sequence), though migration in SDS-PAGE may be around 14 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (amino acids 1-100) of mouse Ly6a, which contains the mature protein sequence after signal peptide cleavage; this design targets the mature polypeptide and may recognize both native and denatured forms.
• For Western blot, consider using whole-cell lysates from mouse tissues or cell lines known to express Sca-1 (e.g., hematopoietic cells), and note that the predicted molecular weight is ~14 kDa, though glycosylation may cause slight shifts.
• In ELISA, the antibody may be suitable for detecting recombinant Ly6a or cell-surface expression; consider testing in direct or indirect ELISA formats with appropriate controls.

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