LSAMP Rabbit Polyclonal Antibody for WB, ELISA - Q13449

Core Product Specifications and Parameters

Biological Background: LSAMP Function and Localization

• LSAMP (Limbic system-associated membrane protein), also known as IgLON family member 3, is a cell adhesion molecule with three immunoglobulin domains and a GPI-anchor.
• It mediates selective neuronal growth and axon targeting, contributing to the guidance of developing axons and remodeling of mature circuits in the limbic system.
• LSAMP is essential for normal growth of the hippocampal mossy fiber projection.
• The protein localizes to the cell membrane, consistent with its role in cell adhesion and axon guidance.
• It is expressed in limbic neurons and fiber tracts, as well as in specific layers of the superior colliculus, spinal cord, and cerebellum.
• LSAMP contains key structural features including disulfide bonds, glycosylation sites, and a GPI-anchor, and it undergoes phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 219-315, a region within the extracellular domain. This selection may provide specificity for LSAMP, but cross-reactivity with other IgLON family members should be verified.
• For Western blotting, the expected band is around 37 kDa; however, due to glycosylation, the observed molecular weight may be higher. Consider using appropriate blocking agents.
• In ELISA, the antibody may be used as a capture or detection reagent; optimize coating and detection conditions using recombinant LSAMP or cell lysates.
• This antibody detects human, mouse, and rat LSAMP, suggesting conserved epitopes; validate in the relevant sample system to confirm reactivity.
• Since LSAMP is GPI-anchored, it may be shed or present in soluble forms; additional steps such as membrane enrichment may improve detection in Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For neuroscience and neuronal guidance research, we supply validated antibody pairs, optimized polyclonal antibodies like this LSAMP antibody, and bulk ancillary reagents. Our sourcing network can also assist with rare target raw-material procurement, covering every stage from concept to clinic.