LRG1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q91XL1

Core Product Specifications and Parameters

Biological Background: LRG1 Function and Localization

• Protein Identity: LRG1 (leucine-rich alpha-2-glycoprotein 1) is encoded by the Lrg1 gene in mouse. Alternative names include A2GL, lrhg, Lrg, 1300008B03Rik, and 2310031E04Rik.
• Structural Features: The protein contains a signal peptide and several leucine-rich repeat (LRR) domains, classifying it as a member of the LRR superfamily. LRR motifs typically form horseshoe-shaped solenoid structures involved in protein–protein interactions.
• Subcellular Localization: The presence of a signal peptide suggests that LRG1 is targeted to the secretory pathway and likely functions in the extracellular space or on the cell surface.
• Functional Insight: While the specific biological role of mouse LRG1 is not yet fully characterized, LRR-containing proteins are commonly implicated in processes such as cell adhesion, signal transduction, and innate immunity. In some organisms, LRG1 is associated with angiogenesis and inflammation.
• Proteomics Data: The protein has been identified in mass spectrometry–based proteomics studies, indicating its expression in certain mouse tissues or cell types.
• UniProt Status: The entry Q91XL1 is currently unreviewed (TrEMBL), meaning that functional annotations are computationally predicted and await experimental validation.

Experimental Guidance and Technical Tips

• The immunogen used for antibody generation consists of a recombinant protein corresponding to amino acids 32–342 of mouse LRG1, which covers the majority of the mature protein and likely recognizes multiple epitopes.
• As a polyclonal antibody, it may exhibit batch-to-batch variability; it is advisable to validate performance in the specific application and sample system of interest.
• The antibody is reported to work in Western blot (WB), immunohistochemistry on paraffin sections (IHC-P), and ELISA. For IHC-P, antigen retrieval conditions may need optimization depending on tissue type.
• Cross-reactivity with human LRG1 is indicated; if using on human samples, consider that the immunogen is based on the mouse sequence (83% amino acid identity between mouse and human orthologs), so binding affinity to human protein may differ.
• Note the theoretical molecular weight of the unprocessed precursor is ~37.4 kDa (UniProt), but the supplier lists a protein weight of 38 kDa, possibly reflecting post-translational modifications or the processed form.
• As with any research antibody, appropriate negative and positive controls should be included to confirm specificity, especially for new experimental settings.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials and technical support, from concept to clinic. This LRG1 polyclonal antibody is part of our extensive portfolio for studying leucine-rich repeat proteins in inflammation and disease. In addition to high-quality antibodies, we can supply validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and sourcing for rare-target raw materials—all designed to streamline your diagnostic development workflow.