LPIN2 Rabbit Polyclonal Antibody for Western Blot and ELISA - Q92539

Core Product Specifications and Parameters

Biological Background: LPIN2 Function and Localization

• LPIN2, also known as Lipin-2, is a magnesium-dependent phosphatidate phosphatase enzyme that catalyzes the conversion of phosphatidic acid to diacylglycerol, essential for triglyceride, phosphatidylcholine, and phosphatidylethanolamine biosynthesis.
• The protein controls fatty acid metabolism at multiple levels and also acts as a nuclear transcriptional coactivator for PPARGC1A, modulating lipid metabolism.
• Subcellularly, LPIN2 is localized to the nucleus, cytoplasm, cytosol, and endoplasmic reticulum membrane, aligning with its dual roles in lipid synthesis and transcription regulation.
• Tissue expression is broad, including liver, lung, kidney, placenta, spleen, thymus, lymph node, prostate, testes, small intestine, and colon.
• Post-translational modifications include phosphorylation, as indicated by the keyword "Phosphoprotein," suggesting regulatory mechanisms.
• Mutations in LPIN2 are associated with congenital dyserythropoietic anemia and hereditary hemolytic anemia, highlighting its clinical relevance.
• Relevant UniProt keywords: Congenital dyserythropoietic anemia, Cytoplasm, Disease variant, Endoplasmic reticulum, Fatty acid metabolism, Hereditary hemolytic anemia, Hydrolase, Lipid metabolism, Magnesium, Membrane, Metal-binding, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 180-400 of human LPIN2, targeting the central region. Consider epitope accessibility when using the antibody in different applications.
• For Western blot, expect a band near 99 kDa. Optimize protein extraction and loading controls for your sample type.
• Validate the antibody in your experimental system using appropriate positive and negative controls. Cross-reactivity with mouse LPIN2 is expected but should be confirmed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, and this LPIN2 polyclonal antibody is a key reagent for lipid metabolism and metabolic disease research. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your diagnostic development from concept to clinic. Let us streamline your procurement with tailored solutions.