LOX Rabbit mAb for WB, IHC-P, IP, ELISA - P28300

Core Product Specifications and Parameters

Biological Background: LOX Function and Localization

• LOX (Protein-lysine 6-oxidase / Lysyl oxidase) is a copper-dependent amine oxidase that catalyzes the post-translational oxidative deamination of peptidyl lysine residues in collagen and elastin precursors, a critical step for the formation of stable extracellular matrix crosslinks. Related references: PMID:26838787

• Beyond its enzymatic role in matrix maturation, LOX acts as a regulator of Ras expression and has been implicated in tumor suppression, highlighting its dual function in both structural and signaling pathways.

• LOX contributes to the structural integrity of the aortic wall, with defects in its function associated with aortic aneurysm pathogenesis.

• The protein is synthesized as a secreted preproprotein, undergoing proteolytic processing to yield the active enzyme, and localizes to the extracellular space after secretion.

• Tissue expression of LOX is broad, with notable presence in heart, placenta, skeletal muscle, kidney, lung, and pancreas.

• Key post-translational modifications include the formation of LTQ (lysine tyrosylquinone) and TPQ (trihydroxyphenylalanine quinone) cofactors essential for catalytic activity, copper binding, disulfide bond formation, and glycosylation, classifying it as a member of the lysyl oxidase gene family.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to a peptide within amino acids 318–417 of human LOX. For WB, a band near 47 kDa is expected for full-length LOX; additional bands representing pro-forms or processed mature enzyme may be observed depending on sample preparation. Consider enriching for extracellular matrix or secreted proteins from cultured cell media if endogenous expression in whole-cell lysates is low.

• For IHC-P, optimization of antigen retrieval conditions is recommended, and strong staining may be seen in connective tissue-rich organs such as aorta, skin, or fibrotic tissues where LOX is actively deposited.

• This antibody is also suitable for IP and ELISA; ensure buffer compatibility for the secreted nature of LOX and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-quality raw-material antibodies for IVD manufacturers and research laboratories. This LOX antibody supports research into fibrotic diseases, cancer biology, and extracellular matrix disorders. As your one-stop sourcing partner, CamelBio provides validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, helping you accelerate development from concept to clinical application.