Lipocalin-2/NGAL Rabbit pAb - P80188

Core Product Specifications and Parameters

Biological Background: Lipocalin-2/NGAL Function and Localization

• Neutrophil gelatinase-associated lipocalin (NGAL), also known as Lipocalin-2, 24p3, or siderocalin, is a secreted glycoprotein belonging to the lipocalin superfamily.
• Functions as an iron-trafficking protein: binds iron through association with bacterial siderophores (e.g., enterobactin) or 2,3-dihydroxybenzoic acid; holo- form increases intracellular iron, while apo- form decreases it, triggering apoptosis via SLC22A17 receptor (PubMed:12453413, PubMed:20581821, PubMed:27780864). Related references: PMID:12453413 , PMID:20581821 , PMID:27780864
• Plays a critical role in innate immunity by sequestering iron-bound microbial siderophores, thereby limiting bacterial and mycobacterial proliferation (PubMed:27780864, PubMed:15642259, PubMed:21978368). Related references: PMID:27780864 , PMID:15642259 , PMID:21978368
• Subcellular locations include secreted, cytoplasmic granule lumen, and cytoplasmic vesicle lumen, consistent with extracellular and intracellular iron handling.
• Highly expressed in bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, lung, and kidney medullary tubules; detected at protein level in neutrophils (PubMed:7683678, PubMed:8298140, PubMed:9339356).
• Contains disulfide bonds and is glycosylated; alternative splicing produces multiple isoforms.
• Not found in small intestine or peripheral blood leukocytes (PubMed:9339356).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 21–198, which excludes the signal peptide, enhancing recognition of the mature secreted form.
• For Western blot, a band around 23–25 kDa is expected; glycosylation may cause slight mobility shifts. Validate in the relevant sample system.
• For IHC-P, consider antigen retrieval methods as the protein is secreted and may require optimization in paraffin-embedded tissues.
• The antibody cross-reacts with mouse; confirm specificity in mouse models if needed.
• For ELISA, use as a capture or detection antibody, but pairing with a monoclonal antibody may be necessary for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality raw materials for targets like Lipocalin-2/NGAL—a key biomarker in renal injury, inflammation, and iron metabolism. As your one-stop sourcing partner, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate your assay development from concept to clinic. From rare target sourcing to technical consultation, CamelBio supports every stage of your IVD workflow.