Lipin 1 Rabbit mAb for WB and ELISA - Q14693

Core Product Specifications and Parameters

Biological Background: Lipin 1 Function and Localization

• Lipin 1 is a magnesium-dependent phosphatidate phosphatase enzyme that catalyzes the conversion of phosphatidic acid to diacylglycerol, a critical step in the biosynthesis of triglycerides, phosphatidylcholine, and phosphatidylethanolamine. Related references: PMID:20231281, PMID:23426360, PMID:29765047
• It is involved in adipocyte differentiation.
• Lipin 1 is recruited to the mitochondrial outer membrane and participates in mitochondrial fission through the local conversion of phosphatidic acid to diacylglycerol.
• It also acts as a nuclear transcriptional coactivator for the PPARGC1A/PPARA regulatory pathway, modulating the expression of genes involved in lipid metabolism.
• Lipin 1 is primarily localized in the cytoplasm and cytosol, and is also associated with the endoplasmic reticulum and nuclear membranes.
• It is specifically expressed in skeletal muscle and adipose tissue, with lower expression in parts of the digestive tract.
• Additional keywords indicate that Lipin 1 undergoes acetylation, alternative splicing, and ubiquitin conjugation, and is involved in fatty acid and lipid metabolism, transcriptional regulation, and metal binding.

Experimental Guidance and Technical Tips

• The immunogen covers a region within amino acids 101–200 of human Lipin 1; for WB, consider using whole cell lysates from tissues with high Lipin 1 expression such as skeletal muscle or adipose tissue.
• Validate the antibody in the appropriate sample system, as reactivity has been demonstrated in human, mouse, and rat samples.
• For ELISA applications, the recombinant fusion protein immunogen can serve as a positive control or coating antigen.
• Due to Lipin 1's multiple isoforms from alternative splicing, confirm detection of the expected molecular weight (~99 kDa) under reducing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined sourcing experience for diagnostic manufacturers by providing validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This Lipin 1 antibody supports research into lipid metabolism, adipocyte differentiation, and mitochondrial dynamics, and CamelBio can assist with sourcing complementary raw materials or developing customized IVD components. Contact us to explore our comprehensive portfolio and technical support services.