LIMD1 Rabbit pAb - Q9UGP4

Core Product Specifications and Parameters

Biological Background: LIMD1 Function and Localization

• LIM domain-containing protein 1 (LIMD1) is an adapter/scaffold protein that assembles complexes regulating cell fate, cytoskeletal organization, transcriptional repression, adhesion, differentiation, proliferation, and migration.
• Part of the LIM protein family; contains LIM domains which are zinc-binding motifs.
• Positively regulates microRNA-mediated gene silencing and is essential for processing body (P-body) formation and integrity.
• Acts as a hypoxic regulator by bridging prolyl hydroxylases and VHL to promote HIF1A degradation.
• Functions as a transcriptional corepressor for SNAI1/SNAI2-dependent repression of E-cadherin and inhibits E2F-mediated transcription.
• Negatively regulates the Hippo signaling pathway and antagonizes YAP1 phosphorylation; may act as a tumor suppressor by inhibiting cell proliferation.
• Localizes to cytoplasm, nucleus, P-bodies, adherens junctions, and focal adhesions.
• Ubiquitously expressed, including in normal and breast cancer tissues.

Experimental Guidance and Technical Tips

• For Western blot, expect a band at approximately 72 kDa; use whole cell lysates from human or mouse cell lines expressing LIMD1.
• Validate specificity with knockdown or knockout controls, as this polyclonal antibody may recognize multiple epitopes.
• For ELISA, consider pairing with a suitable detection antibody; optimize coating concentration and detection conditions.
• The immunogen corresponds to the N-terminal region (aa 1-200), so it may not detect isoforms lacking this region; verify in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, offering validated polyclonal antibodies like this anti-LIMD1 antibody for cancer research and cell signaling studies. We supply bulk ancillary reagents, optimized antibody pairs, and rare target raw materials to support your diagnostic development. Contact us for customized monoclonal/polyclonal antibody services and technical consultation to bridge your project from concept to clinic.