LHX2 Rabbit mAb for WB and ELISA - P50458

Core Product Specifications and Parameters

Biological Background: LHX2 Function and Localization

• LHX2 is a LIM/homeobox transcription factor that functions as a transcriptional activator.
• It stimulates the promoter of the alpha-glycoprotein gene, indicating a role in glycoprotein hormone regulation.
• LHX2 is involved in the control of cell differentiation, particularly in developing lymphoid and neural cell types.
• The protein contains LIM domains (zinc-binding motifs) and a homeobox DNA-binding domain, classifying it as a member of the LIM-homeodomain family.
• It localizes to the nucleus, consistent with its function as a DNA-binding transcription factor.
• Zinc binding is crucial for the structural integrity of the LIM domains.
• Keywords from UniProt: activator, DNA-binding, homeobox, LIM domain, metal-binding, transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human LHX2; consider using peptides for blocking or competition assays to confirm specificity.
• For Western blot, test in nuclear extracts or whole cell lysates from human, mouse, or rat lymphocytes, neural tissues, or relevant cell lines (e.g., Jurkat, SH-SY5Y). The predicted molecular weight is ~44 kDa, but confirm with known positive controls.
• In ELISA applications, consider using recombinant LHX2 protein or peptide as standard, and optimize coating conditions and antibody dilutions.
• Cross-reactivity with mouse and rat LHX2 is annotated; however, verify performance in your specific sample system, as sequence homology may influence binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined gateway to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For transcription factor research and lymphoid/neural development studies, CamelBio provides consistent-access reagents like this LHX2 monoclonal antibody, supported by flexible customization and rare-target sourcing services.