LGALS2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P05162

Core Product Specifications and Parameters

Biological Background: Galectin-2 Function and Localization

• Galectin-2 (LGALS2), also referred to as HL14, beta-galactoside-binding lectin L-14-II, lactose-binding lectin 2, or S-Lac lectin 2, is a member of the galectin family of carbohydrate-binding proteins.
• The protein binds beta-galactoside sugars, suggesting a role in carbohydrate recognition and cellular interactions.
• Its precise physiological function remains unknown based on current annotations.
• Structural studies have been reported, as indicated by the 3D-structure keyword.
• Galectin-2 has been identified through proteomics approaches, confirming its expression in human samples.
• It is part of the UniProt reference proteome, ensuring high-quality sequence and functional annotation.

Experimental Guidance and Technical Tips

• The immunogen consists of the full-length LGALS2 protein (residues 1-74), enabling detection of the native conformation or linear epitopes across the entire sequence.
• Given the predicted molecular weight of 15 kDa, a standard SDS-PAGE protocol with appropriate molecular weight markers is recommended for Western blot analysis.
• For immunofluorescence, optimize fixation and permeabilization conditions based on the sample type, as galectin-2 localization may be cytosolic or secreted.
• ELISA performance can be confirmed using recombinant LGALS2 as a positive control, ensuring specificity for the target antigen.
• Validate cross-reactivity in mouse samples by comparing with a known positive control for consistent reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering validated antibodies and ancillary reagents for diagnostic development. This LGALS2 antibody supports research into lectin biology and carbohydrate-mediated processes, and we can further provide optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk raw materials, and customized sourcing for rare targets to meet your project needs from concept to clinic.