LECT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75829

Core Product Specifications and Parameters

Biological Background: LECT1/CNMD Function and Localization

• LECT1 (CNMD) encodes a bifunctional growth regulator known as chondromodulin, which stimulates chondrocyte growth but inhibits endothelial cell proliferation and tube formation, playing a key role in endochondral bone development.
• Alternative names include Chondromodulin, CHM1, CHM-I, BRICD3, and MYETS1; the protein exists in secreted and transmembrane forms due to alternative splicing.
• The protein contains a transmembrane helix (in some isoforms) and is primarily localized to the extracellular space, extracellular matrix, and endomembrane system.
• LECT1 is highly expressed in cartilage and cardiac valves (laminae fibrosa, spongiosa, and ventricularis layers), with decreased expression in diseased valves; weakly expressed in chondrosarcoma.
• Post-translational modifications include cleavage on pair of basic residues and disulfide bond formation; it is glycosylated.
• Key functional annotations involve chondrogenesis, developmental processes, differentiation, and extracellular matrix organization; it is listed as a secreted and transmembrane protein.
• Acts as an antiangiogenic factor in cardiac valves, suppressing neovascularization, which is crucial for maintaining valve homeostasis.
• Its molecular weight is approximately 37 kDa (UniProt: 37102 Da), matching the expected band in immunoblotting.

Experimental Guidance and Technical Tips

• The immunogen (aa 75–334) covers a central region of the protein, potentially recognizing both full-length and processed forms; for Western blot, a band at ~37 kDa is expected, and higher bands may indicate disulfide-linked dimers.
• Reduction with DTT or beta-mercaptoethanol may resolve multimeric species; validate the antibody in relevant tissue lysates (e.g., cartilage, cardiac valves) to confirm specificity.
• For immunofluorescence/ICC, permeabilization with Triton X-100 or similar is recommended to detect intracellular epitopes, as LECT1 can be secreted or membrane-associated.
• In ELISA, use recombinant protein or peptide controls to assess sensitivity and specificity; titration and proper blocking are advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with one-stop access to high-quality IVD raw materials, from concept to clinic. This anti-LECT1/Chondromodulin antibody supports research in cartilage development, angiogenesis, and cardiac valve biology. Beyond antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.