LDL Receptor (LDLR) Rabbit Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P01130

Core Product Specifications and Parameters

Biological Background: LDL Receptor (LDLR) Function and Localization

• The low-density lipoprotein receptor (LDLR) is a cell-surface glycoprotein that mediates the endocytosis of cholesterol-rich LDL particles, playing a central role in systemic cholesterol homeostasis.
• LDLR binds LDL and facilitates its internalization via clathrin-coated pits; efficient internalization is promoted through the formation of a ternary complex with PGRMC1 and TMEM97. Related references: PMID:30443021
• Beyond its canonical role in cholesterol metabolism, LDLR acts as a host receptor for multiple viruses, including hepatitis C virus (HCV), vesicular stomatitis virus, HIV-1 (via Tat protein), Crimean-Congo hemorrhagic fever virus, and several alphaviruses (GETV, RRV, Semliki Forest virus).
• The receptor contains several distinct domains: an N-terminal ligand-binding region with LDL-receptor class A repeats, an EGF-like domain, a transmembrane helix, and a C-terminal intracellular tail that interacts with adaptor proteins for coated pit localization.
• LDLR cycles between the plasma membrane and endocytic compartments; it is found at the cell surface, in clathrin-coated pits, Golgi apparatus, early and late endosomes, and lysosomes.
• Post-translational modifications include N-linked glycosylation, disulfide bond formation, phosphorylation, and ubiquitination, all of which can influence receptor stability and trafficking.
• Mutations in the LDLR gene cause familial hypercholesterolemia (FH), a genetic disorder characterized by elevated plasma LDL cholesterol and premature cardiovascular disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 761–860 of human LDLR (NP_000518.1), which maps to the intracellular C‑terminal domain. For immunofluorescence detection, permeabilization of cells is recommended to allow antibody access.
• In Western blotting, a specific band at approximately 95 kDa (precursor form) is observed, though additional bands representing processed or degraded species may be present depending on sample preparation and cell type.
• For immunohistochemical staining of paraffin sections, an antigen retrieval step may improve epitope accessibility. Validate the antibody in the relevant species and tissue system before use.
• As a rabbit monoclonal antibody, it is suitable for multiplexing with mouse primary antibodies in co‑staining experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a seamless, one‑stop pathway to critical IVD raw materials like this LDL Receptor antibody. Whether you need validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, or rare‑target sourcing, our integrated supply network supports your journey from concept to clinic. For projects focused on cholesterol metabolism, cardiovascular disease biomarkers, or viral entry mechanisms, we are ready to help you secure the right materials with confidence.