LAMTOR5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43504

Core Product Specifications and Parameters

Biological Background: LAMTOR5 Function and Localization

• LAMTOR5, also known as HBXIP or XIP, is a component of the Ragulator complex, which plays a critical role in amino acid sensing and mTORC1 activation. Related references: PMID:22980980, PMID:29158492, PMID:30181260
• As part of the Ragulator, LAMTOR5 functions as a guanine nucleotide exchange factor (GEF) for the small GTPases Rag and mediates their recruitment to the lysosomal membrane. Related references: PMID:22053050, PMID:22980980, PMID:28935770
• Activated Ragulator and Rag GTPases serve as a scaffold to recruit mTORC1 to lysosomes, where mTORC1 is subsequently activated. Related references: PMID:22980980, PMID:29158492, PMID:30181260
• When complexed with BIRC5 (survivin), LAMTOR5 interferes with apoptosome assembly, preventing recruitment of pro-caspase-9 to oligomerized APAF1, thereby suppressing mitochondrial/cytochrome c-mediated apoptosis. Related references: PMID:12773388
• LAMTOR5 localizes primarily to lysosomes, but is also found in the cytoplasm and cytosol.
• Tissue expression is highest in skeletal and cardiac muscle, with lower levels in pancreas, kidney, liver, brain, placenta, and lung. Expression is elevated in liver tissue from patients with chronic HBV infection. Related references: PMID:9499022
• Post-translational modifications include acetylation, and the protein has been identified through direct protein sequencing and proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human LAMTOR5 protein (aa 1-173), ensuring recognition of the native target. Consider confirming specificity using knockout or knockdown controls.
• Recommended applications include WB, IF/ICC, and ELISA. For WB, use a 15% or gradient gel to resolve the low-molecular-weight band (~10 kDa).
• Cross-reactivity has been reported with human, mouse, and rat; validate in the relevant sample system if working with other species.
• Since this is a polyclonal antibody, lot-to-lot consistency should be monitored, and optimal working dilutions should be determined empirically for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop sourcing platform for IVD raw materials, from initial target discovery to clinical application. Our offerings include validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored support for rare targets such as LAMTOR5. With expertise in mTOR signaling and apoptosis research, we empower your diagnostic assay development with robust, scalable raw material solutions.