Lamin B2 Rabbit Monoclonal Antibody for WB - Q03252

Core Product Specifications and Parameters

Biological Background: Lamin B2 Function and Localization

• Lamin B2 is an intermediate filament protein that constitutes the major component of the nuclear lamina, a meshwork lining the inner nuclear membrane. Related references: PMID:33033404
• It plays an essential role in nuclear assembly, chromatin organization, and nuclear membrane and telomere dynamics, bridging the nuclear envelope and chromatin. Related references: PMID:33033404
• Localized to the nucleus lamina, it is key to maintaining nuclear structural integrity.
• The protein undergoes various post-translational modifications including acetylation, phosphorylation, methylation, and prenylation, which regulate its function.
• Mutations in the LMNB2 gene are associated with neurological disorders such as primary microcephaly, epilepsy, and intellectual disability, highlighting its critical role in brain development.

Experimental Guidance and Technical Tips

• For Western blotting, the expected molecular weight of Lamin B2 is approximately 70 kDa; consider optimizing gel percentage and transfer conditions accordingly.
• In immunohistochemistry and immunofluorescence, validate staining patterns in appropriate human, mouse, or rat tissues or nuclear preparations, as the antibody cross-reacts with these species.
• The immunogen is a synthetic peptide from amino acids 521–620; verify specificity by comparing signals with expected isoform or splice variant expression in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive one-stop sourcing platform for IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For nuclear lamina and cell cycle research, we provide reliable access to anti-Lamin B2 antibodies suitable for multiple applications. Our supply capabilities extend to rare target raw-material sourcing, supporting your projects from concept to clinic.