Lamin B1 Rabbit mAb for WB, IHC-P - P20700

Core Product Specifications and Parameters

Biological Background: Lamin B1 Function and Localization

• Lamin-B1, encoded by the LMNB1 gene, is a type V intermediate filament protein and a core component of the nuclear lamina.
• It forms a filamentous meshwork that provides a structural scaffold for the nuclear envelope, bridging the nuclear lamina and chromatin, and is essential for nuclear assembly, chromatin organization, and nuclear membrane and telomere dynamics. Related references: PMID:28716252, PMID:32910914
• The lamin meshwork undergoes cell cycle-dependent disassembly and reassembly, disintegrating in prophase and reforming in telophase.
• Lamin-B1 plays a specific role in spermiogenesis, anchoring the sperm head to the tail via the LMNB1/SUN5/SEPT12 bridge complex. Related references: PMID:38870534
• The protein is subject to multiple post-translational modifications, including acetylation, phosphorylation, methylation, and prenylation, which regulate its function and dynamics.
• Mutations in LMNB1 are associated with autosomal dominant leukodystrophy, primary microcephaly, and intellectual disability.
• Subcellular localization is restricted to the nuclear lamina.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 450–550 of human Lamin-B1 (P20700); considering this, the antibody may recognize a C‑terminal epitope.
• For Western blot (WB), consider using whole‑cell lysates from human, mouse, or rat cell lines; the predicted molecular weight is ~66 kDa, so use appropriate pre‑stained markers.
• For immunohistochemistry (IHC‑P), standard antigen retrieval techniques may be needed; validate signal specificity with appropriate nuclear and negative controls.
• As a nuclear envelope protein, subcellular fractionation could help enrich the target signal in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one‑stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For nuclear lamina and chromatin biology research, we supply Lamin B1 antibodies in flexible quantities and can assist with rare‑target raw‑material sourcing, helping you accelerate diagnostic development from concept to clinic.