Lactylated Histone H3-K18 Rabbit Monoclonal Antibody (Q16695/P68431) for Epigenetic Research

Core Product Specifications and Parameters

Biological Background: Histone H3 (Lactylated K18) Function and Localization

• Histone H3 is a core component of the nucleosome, wrapping and compacting DNA into chromatin to regulate DNA accessibility for transcription, repair, replication, and chromosomal stability.
• The protein localizes to the nucleus and chromosomes, with tissue-specific expression patterns: the H3.1t isoform (Q16695) is predominantly expressed in testicular cells, while the H3.1 isoforms (P68431) are widely expressed.
• Histone H3 undergoes extensive post-translational modifications (PTMs), including acetylation, methylation, phosphorylation, and lactoylation, collectively known as the histone code, which modulates chromatin dynamics and gene expression.
• Lactoylation of histone lysine residues, such as at K18, has emerged as an important modification linking cellular metabolism to epigenetic regulation. This rabbit monoclonal antibody specifically recognizes lactic acid-modified lysine 18 on histone H3.
• Lactoylation at the adjacent K19 residue has been documented (PMID: 31645732), highlighting the potential functional significance of this region.

Related references:
UniProt Q16695
UniProt P68431
PMID:31645732

Experimental Guidance and Technical Tips

• For Western blot, consider using a low-percentage SDS-PAGE gel (e.g., 10-12%) to resolve the ~15 kDa histone H3 protein, and optimize antibody dilution based on the expression level of the lactylated form in your sample.
• Employ appropriate negative controls, such as a non-modified peptide competitor or untreated cell lysates with low lactylation, to confirm specificity.
• In ELISA, coat with the lactylated peptide and validate against a non-lactylated control to assess cross-reactivity.
• Since lactoylation can be induced under specific metabolic conditions (e.g., high lactate), consider treating cells with relevant stimuli and validating in the relevant sample system.
• Note that the antibody is predicted to have wide cross-reactivity (human, mouse, rat, and others), but validation in the desired species is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This lactylated histone H3-K18 antibody supports research into epigenetic modifications and metabolic-epigenetic crosstalk, and we offer end-to-end sourcing solutions from target discovery to clinical application. For rare or custom modification-specific antibodies, we leverage our network to help you secure the raw materials you need, backed by technical consultation and reliable supply chains.