Lactic acid-Histone H3-K18 Rabbit Polyclonal Antibody (Q16695/P68431) for WB, DB, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

Histone H3 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Nucleosomes compact DNA, regulating accessibility to the transcriptional, repair, and replication machineries. Through a dynamic array of post‑translational modifications (PTMs)—collectively termed the histone code—histone H3 plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Related references: UniProt Q16695, UniProt P68431

Histone H3 localizes to the nucleus and is tightly associated with chromosomal DNA. Its presence on nucleosome cores positions it as a structural and regulatory platform for chromatin‑based processes.
Related references: UniProt Q16695, UniProt P68431

Multiple histone H3 variants exist. The canonical H3.1 (encoded by several genes including H3C1, H3C2, etc., UniProt P68431) is expressed broadly, whereas H3.1t (H3‑4, UniProt Q16695) exhibits testis‑specific expression. The amino‑acid sequence surrounding K18 is highly conserved among these variants, supporting the wide predicted species and variant cross‑reactivity of this antibody.
Related references: UniProt Q16695 (tissue specificity), UniProt P68431

Lysine 18 of histone H3 is a hot‑spot for several PTMs. It undergoes asymmetric dimethylation catalyzed by CARM1 and can also be citrullinated. These modifications participate in transcriptional activation, chromatin remodeling, and epigenetic signaling.
Related references: PMID:15345777, PMID:15471871, PMID:16497732, PMID:16567635

Experimental Guidance and Technical Tips

• Because this antibody was generated against a lactylated peptide, consider performing peptide‑competition assays to verify its specificity for lactylated H3K18 versus the unmodified peptide or other PTMs.
• For Western blotting, standard acid‑extraction protocols for histones can be employed. Optimize blocking conditions and primary antibody dilution in your particular sample matrix.
• In ELISA‑based assays, coat with the appropriate lactylated peptide. Include non‑lactylated and irrelevant PTM peptides as negative controls to assess binding specificity.
• Although broad cross‑reactivity is predicted across human, mouse, and rat samples, always validate performance in your own experimental system with proper loading and PTM controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD manufacturers and research laboratories, providing a seamless supply of specialized raw materials—from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. For projects involving epigenetic targets such as histone lactylation, we facilitate access to rare‑specificity antibodies, tailored sourcing solutions, and technical consultation to help bridge the gap from concept to clinic.