L3MBTL3 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q96JM7

Core Product Specifications and Parameters

Biological Background: L3MBTL3 Function and Localization

• L3MBTL3 (Lethal(3)malignant brain tumor-like protein 3), also known as MBT-1, is a member of the malignant brain tumor (MBT) family and contains zinc-finger domains characteristic of chromatin regulators.

• It functions as a negative regulator of Notch target gene expression by recruiting the lysine-specific demethylase KDM1A (LSD1) to Notch-responsive elements, promoting H3K4me1/2 demethylation and transcriptional repression.
  Related references: PMID:29030483

• L3MBTL3 is involved in ubiquitin-dependent degradation of methylated non-histone proteins, including SOX2, DNMT1, and E2F1. It acts as an adapter, linking methylated substrates to the CRL4-DCAF5 E3 ubiquitin ligase complex for proteasomal degradation.
  Related references: PMID:29691401 | PMID:30442713

• The protein is required for normal maturation of myeloid progenitor cells, although the precise mechanism remains to be fully elucidated.

• Localization is predominantly nuclear, consistent with its roles in transcriptional regulation and chromatin modification.

• Post-translational modifications include phosphorylation and ubiquitin-like conjugation (isopeptide bond formation), which may regulate its activity and interactions.

• The gene undergoes alternative splicing, potentially generating isoforms with distinct functional properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–255 at the N-terminus of human L3MBTL3. Antibody binding is likely directed to epitopes within this region; verify specificity using recombinant protein or peptide competition assays if needed.

• For Western blot (WB), anticipate a specific band near 88 kDa, though actual migration can be influenced by post-translational modifications; validate with appropriate positive and negative controls.

• In immunoprecipitation (IP) experiments, optimize antibody concentration and lysis buffer conditions to preserve protein interactions; test performance in your specific experimental system.

• For ELISA applications, perform antibody titration and use a matched detection system. Note that cross-reactivity with non-human species has not been determined—validate if using samples from other organisms.

• This product has been validated on human samples; for studies on other species, assess cross-reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material supplier, CamelBio connects diagnostic developers and researchers with critical reagents like this anti-L3MBTL3 monoclonal antibody. Our epigenetics and chromatin regulation portfolio supports assay development for cancer and stem cell research. Beyond individual antibodies, we offer one-stop sourcing for validated antibody pairs, bulk reagents, and custom procurement to streamline your workflow from concept to clinical application.