KRAS+HRAS+NRAS Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P01111 / P01112 / P01116

Core Product Specifications and Parameters

Biological Background: Pan-Ras (KRAS/HRAS/NRAS) Function and Localization

• The NRas protein is a small GTPase that functions as a molecular switch in the Ras-MAPK signaling pathway, controlling cell proliferation and survival through GDP/GTP cycling and intrinsic GTPase activity (PubMed:30712867). Related references: PMID:30712867

• KRAS, HRAS, and NRAS are members of the classical Ras family, sharing high sequence homology; they regulate diverse cellular processes downstream of growth factor receptors, but each isoform can exhibit distinct functions and tissue-specific expression.

• Subcellularly, NRas localizes to the cell membrane and the Golgi apparatus membrane, where it transmits extracellular signals to intracellular effectors.

• Post-translational modifications, including farnesylation, palmitoylation, and ubiquitination, dynamically control NRas trafficking and stability; for example, LZTR1-mediated ubiquitination marks NRas for proteasomal degradation (PubMed:40934300). Related references: PMID:40934300

• Mutations in NRAS, KRAS, and HRAS that lock the proteins in the GTP-bound state are potent oncogenic events, making pan-Ras antibodies valuable tools for cancer research and diagnostic development.

• The antibody was raised against a conserved N-terminal region (aa 1-100) of human NRAS, which is highly identical among the three isoforms, enabling broad-spectrum detection.

Experimental Guidance and Technical Tips

• Based on the immunogen design targeting the conserved N-terminus of NRAS (aa 1-100), the antibody is expected to detect KRAS, HRAS, and NRAS; however, isoform-specific reactivity should be validated using recombinant proteins or knockout cell lines.
• For WB, a band at approximately 18kDa is observed under reducing conditions; use appropriate loading controls and confirm specificity with siRNA knockdown or overexpression.
• In IF/ICC, consider fixing cells with 4% PFA and permeabilizing with Triton X-100; optimize antibody concentration to minimize background, as membrane-localized targets may require gentle permeabilization.
• For ELISA, the antibody can be paired with appropriate detection reagents; ensure the plate coating captures the antigen in its native conformation for accurate quantification.
• Since Ras signaling is tightly regulated, include controls for post-translational modifications (e.g., farnesylation inhibitors) if investigating membrane translocation.

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CamelBio connects IVD manufacturers and researchers with high-quality raw materials, such as this pan-Ras Rabbit monoclonal antibody, supporting your cancer signaling diagnostic or research workflow. We offer one-stop access to validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare targets—helping you seamlessly progress from assay concept to clinical application.