KPNB1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14974

Core Product Specifications and Parameters

Biological Background: KPNB1 Function and Localization

• Importin subunit beta-1 (KPNB1), also known as karyopherin beta-1 or importin-90, functions as a nuclear transport receptor, mediating protein import either in association with an importin-alpha adapter or autonomously by recognizing nuclear localization signals (NLS) directly. Related references: PMID:10228156, PMID:11682607, PMID:11891849

• The importin/substrate complex docks to the nuclear pore complex (NPC) through KPNB1 binding to nucleoporin FxFG repeats, followed by an energy-dependent, Ran-driven translocation across the nuclear envelope. Related references: PMID:10228156, PMID:11682607, PMID:11891849

• On the nucleoplasmic side, RanGTP binding dissociates the complex, releasing the cargo; importin subunits are then recycled to the cytoplasm for subsequent transport cycles. Related references: PMID:10228156, PMID:11682607, PMID:11891849

• KPNB1 autonomously imports ribosomal proteins RPL23A, RPS7, and RPL5, and cooperates with IPO7 to import histone H1; it can also mediate nuclear import of core histones H2A, H2B, H3, and H4 in vitro. Related references: PMID:11682607, PMID:9687515, PMID:10228156

• Additional cargo includes transcription regulators such as MRTFA, SNAI1, and PRKCI, highlighting its broad role in signal-dependent nuclear transport. Related references: PMID:11891849, PMID:19386897, PMID:20818336

• During HIV-1 infection, KPNB1 binds and mediates nuclear import of the viral Rev protein, facilitating viral replication.

• The protein localizes predominantly in the cytoplasm and at the nuclear envelope, consistent with its function at the nucleocytoplasmic interface.

• KPNB1 undergoes post-translational modifications including acetylation, phosphorylation, and ADP-ribosylation, and alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band near 97 kDa; include appropriate positive controls (e.g., HeLa, HEK293 lysates) and consider using a loading control for normalization.
• In immunohistochemistry (IHC-P), test antigen retrieval methods (e.g., citrate buffer, Tris-EDTA) and titrate antibody dilution to minimize background; a nuclear/cytoplasmic staining pattern is anticipated.
• For immunofluorescence (IF/ICC), optimize fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) conditions, and use a suitable secondary antibody to detect the rabbit primary.
• ELISA applications can be performed with indirect coating of the peptide immunogen or recombinant KPNB1 protein; validate assay conditions with known positive and negative samples.
• Because this is a polyclonal antibody, lot-to-lot consistency should be confirmed when changing batches in long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with ready-to-use IVD raw materials for nuclear transport and cancer biology applications. We supply validated antibody pairs, optimized monoclonal/polyclonal reagents like this KPNB1 antibody, and bulk ancillary reagents, while also offering rare target sourcing and technical consulting to accelerate your assay development from concept to clinic.