[KO Validated] YTHDF2 Rabbit mAb - Q9Y5A9

Core Product Specifications and Parameters

Biological Background: YTHDF2 Function and Localization

• YTHDF2 is an m6A reader protein that specifically binds N6-methyladenosine (m6A)-modified RNAs to regulate their stability and processing. Related references: PMID:24284625, PMID:26046440, PMID:26318451
• It promotes degradation of m6A-containing mRNAs by recruiting the CCR4-NOT deadenylase complex via direct interaction with CNOT1. Related references: PMID:27558897
• In cooperation with RIDA/HRSP12, it directs transcripts bearing a specific binding site (5'-GGUUC-3') toward endoribonucleolytic cleavage by the ribonuclease P/MRP complex. Related references: PMID:30930054
• Upon binding to polymethylated mRNAs, YTHDF2 undergoes liquid-liquid phase separation, driving formation of membraneless compartments such as P-bodies and stress granules. Related references: PMID:31292544, PMID:31388144, PMID:31642031
• Primarily localizes to the cytosol, P-bodies, and stress granules; under heat shock it relocalizes to the nucleus to protect m6A marks and promote cap-independent translation. Related references: PMID:26458103
• Essential for oocyte maturation and spermatogenesis by controlling maternal mRNA decay (By similarity).
• Regulates hematopoietic stem cell specification and inhibits neural differentiation of iPSCs by promoting degradation of m6A-modified target transcripts. Related references: PMID:30065315, PMID:32169943
• Inhibits type I interferon response by binding and degrading m6A-containing IFNB transcripts. Related references: PMID:30559377
• Regulates circadian hepatic lipid metabolism by promoting m6A-dependent degradation of PPARA transcripts. Related references: PMID:30428350
• Highly expressed in induced pluripotent stem cells (iPSCs) and down-regulated during neural differentiation.

Experimental Guidance and Technical Tips

• For WB, a starting dilution of 1:1000 is often effective; however, optimization is recommended. Validate specificity using knockout cell lines or siRNA silencing.
• For IHC-P, perform heat-induced antigen retrieval and consider a dilution range of 1:50-1:200.
• For IP, typical antibody amounts of 1-2 µg per 500 µg total protein may be tested.
• For ELISA, coating with the immunogen peptide or recombinant YTHDF2 is suggested.
• Given the m6A-binding function, consider potential competition with endogenous methylated RNA in IP and other RNA-associated assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, supporting diagnostic manufacturers and research labs with validated antibodies and reagents. Our YTHDF2 monoclonal antibody is part of a comprehensive portfolio for epitranscriptomics research, enabling the development of novel diagnostic assays targeting RNA modifications. From bulk antibody orders to customized reagent sourcing, CamelBio streamlines your procurement process from concept to clinic.