KO Validated XIAP Rabbit Polyclonal Antibody for WB, ELISA - P98170

Core Product Specifications and Parameters

Biological Background: XIAP Function and Localization

• XIAP is a multi-functional protein that directly inhibits apoptosis by binding and blocking the active sites of caspases-3 and -7, and by maintaining caspase-9 in an inactive monomeric state.
  Related references: PMID:11257230 PMID:11257231 PMID:12620238 PMID:16352606

• As an E3 ubiquitin-protein ligase, XIAP catalyzes Lys-63-linked polyubiquitination of RIPK2 downstream of NOD1/2, creating a scaffold for NF-κB and MAP kinase activation, and also ubiquitinates RIPK1, MAP3K2, and other signaling mediators to fine-tune inflammatory and survival pathways.
  Related references: PMID:17560374 PMID:17967870 PMID:19473982 PMID:19667203 PMID:22607974 PMID:29452636

• XIAP participates in copper homeostasis by ubiquitinating COMMD1 for proteasomal degradation and enhancing CCS chaperone activity toward SOD1, thereby regulating cellular copper metabolism.
  Related references: PMID:14685266 PMID:20154138

• It positively regulates Wnt signaling by ubiquitinating TLE transcription co-repressors, leading to their dissociation from TCF/LEF and enabling β-catenin-driven transcriptional programs.
  Related references: PMID:22304967

• XIAP suppresses ripoptosome formation and caspase-8-dependent cell death by ubiquitinating RIPK1 and caspase-8.
  Related references: PMID:22095281

• The protein localizes to both the cytoplasm and the nucleus.

• Ubiquitously expressed, except in peripheral blood leukocytes; detected at the protein level in colonic crypts.
  Related references: PMID:8654366 PMID:30389919

• XIAP belongs to the inhibitor of apoptosis (IAP) family and contains three BIR domains and a C-terminal RING finger domain; it is subject to regulatory phosphorylation, S-nitrosylation, and ubiquitin-like modifications.

Experimental Guidance and Technical Tips

• The immunogen (residues 198‑497) covers the BIR2, BIR3, and part of the RING domain, which are highly conserved across human, mouse, and rat, supporting the observed cross‑reactivity. Consider verifying cross‑reactivity in your specific model system by including appropriate controls.
• For Western blot, a band of approximately 57 kDa is expected; optimize blocking and antibody dilution using a lysate from a cell line with confirmed XIAP expression (e.g., HeLa or Jurkat). Due to KO validation, this antibody may offer high specificity; nonetheless, validate in your own experimental setup.
• While the antibody is listed for ELISA, performance may depend on antigen presentation; consider using recombinant XIAP protein or cell lysates as coating antigen and validate assay conditions.
• XIAP functions in multiple signaling pathways (NF‑κB, Wnt, apoptosis); when studying its role, use appropriate stimuli (e.g., TNFα, NOD1/2 ligands) and inhibitors to dissect pathway-specific effects.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner offering one‑stop access to validated antibodies, recombinant proteins, and ancillary reagents for diagnostic and life‑science research. This XIAP antibody targets a key apoptosis and cancer signaling regulator, and we can supply matched antibody pairs, optimized monoclonal or polyclonal antibodies, and bulk ancillary reagents to support your assay development. Whether you require rare‑target sourcing or custom conjugation, our team connects you with quality materials from concept to clinic.