[KO Validated] β-Catenin Rabbit mAb for WB, IP, IF-P, IHC-P, ELISA - P35222

Core Product Specifications and Parameters

Biological Background: β-Catenin Function and Localization

• β-Catenin is the key downstream effector of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a destruction complex with AXIN, APC, and GSK3B, leading to its phosphorylation, ubiquitination, and proteasomal degradation. Upon Wnt activation, β-catenin accumulates and translocates to the nucleus where it acts as a coactivator for TCF/LEF transcription factors. Related references: PMID:17524503 PMID:18077326 PMID:18086858
• β-Catenin is a component of the E-cadherin:catenin adhesion complex, linking cell adhesion to the actin cytoskeleton at adherens junctions.
• It promotes epithelial to mesenchymal transition (EMT) by driving transcription of CTNNB1/TCF-target genes. Related references: PMID:29910125
• Subcellularly, β-catenin localizes to the cytoplasm, nucleus, cell membrane, adherens junctions, centrosome, spindle poles, and basal body of cilia, reflecting its diverse functions.
• Tissue expression includes hair follicle cells, colon, cortical neurons (at protein level), and is notably detected in breast cancer tissues. Related references: PMID:29367600
• Post-translational modifications, such as N-terminal phosphorylation and ubiquitination, regulate protein stability; acetylation and S-nitrosylation further modulate its activity.
• β-Catenin is implicated in other processes: it negatively regulates centrosome cohesion, facilitates insulin internalization, blocks anoikis in malignant cells, and disrupts PML-NB formation. Related references: PMID:18086858 PMID:21262353 PMID:18957423 PMID:22155184

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa1–100) of human β-catenin, which contains critical residues for phosphorylation-dependent degradation. Consider using the antibody to monitor total β-catenin levels versus phosphorylated forms.
• For Western blotting, the expected band is at ∼85 kDa. Validate expression in your sample system, as β-catenin is widely expressed but subject to regulated turnover.
• For immunohistochemistry (paraffin), include appropriate antigen retrieval steps and control for membrane, cytoplasmic, and nuclear localization.
• The antibody is suitable for detecting β-catenin across human, mouse, and rat. Validate cross-reactivity in your target species.
• KO validation confirms on-target recognition; however, as with any antibody, performance may vary by application and sample type—independent validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers a streamlined sourcing solution for IVD raw materials. This anti-β-Catenin rabbit monoclonal antibody supports Wnt signaling and cell adhesion research. CamelBio can supply validated antibody pairs, optimized detection reagents, and ancillary materials to accelerate your assay development. For custom or bulk orders, our team bridges the gap from concept to clinic.